Proximity Ligation Assay – Reported in literature 2 RRID Number AB_2796300 Documentation Technical Bulletin Safety Datasheet Certificate of Analysis Lookup Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view Cat. No. Lot. No. Need help with this product?
As expected, the ΔbioBFDA mutant cannot appear on the M9 minimal medium without biotin in this biotin assay (Fig. 1b). The growth defection of this mutant was significantly restored by the exogenous addition of biotin (2–10 pmol, Fig. 1b), rather than its precursor DTB (Fig. 1c)....
Requirement of biotin forA. tumefaciensgrowth ThebioBFDAoperon ofA. tumefaciensencodes four enzymes responsible for the latter steps of biotin synthesis, assuring the physiological requirement of biotin (Fig.1a). Presumably, the removal of this operon impairs its capability of biotin synthesis, giving ...