flow cytometryproximity ligation assayExtracellular vesicles (EVs) are continuously released by most cells, and they carry surface markers of their cells of origin. Found in all body fluids, EVs function as conveyers of cellular information, and evidence implicates them as markers of disease. These ...
邻近连接分析,英文Proximity ligation assay,简称PLA, 中文翻译为邻位连接分析或者邻近连接分析技术。 邻近连接分析PLA是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。 邻近连接分析PLA由Fredriksson等人于2002年开发。到目前为止,它已成为一种成熟且普遍适用的免疫组化工具,用于高级和精确的蛋白质分析。
Based on the in situ proximity ligation assay (in situ PLA), proximal oligonucleotide-conjugated antibodies bound to their targets on the surfaces of the EVs allow formation of circular products that can be fluorescently labeled by rolling circle amplification. The intense fluorescent signals produced ...
全称为Proximity Ligation Assay 是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多克隆抗体的探针,即PLA probe(探针),识别目的蛋白,当这两个探针识别同一个蛋白时,两个探针之间的距离靠近,产生了所谓的邻近效应(proximity)。
ProteinSeq: High-Performance Proteomic Analyses by Proximity Ligation and Next Generation Sequencing We present herein a multiplex proximity ligation assay with readout via realtime PCR or DNA sequencing (ProteinSeq). We demonstrate improved sensitivity ... S Darmanis,RY Nong,J Vänelid,... - ...
邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 发布邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 剧情介绍:
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irus-Infected Cells Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected CellsProximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected CellsColocalization...
PROXIMITY LIGATION ASSAY 专利名称：PROXIMITY LIGATION ASSAY 发明人：Matthew Levy,Andrew D. Ellington,Supriya Pai 申请号：US11834546 申请日：20070806 公开号：US20080293051A1 公开日：20081127 专利内容由知识产权出版社提供 专利附图：摘要：The present invention relates to compositions and methods for sensitive...