The proximity ligation assay (PLA) is a sensitive and specific technique that uses sets of proximity probes to induce the formation of circular DNA strands when the probes are bound in close proximity. The DNA circles serve as templates for amplification reactions allowing the localized detection ...
We recently demonstrated that WRAP53 acts as a key regulator of ubiquitin-dependent repair of DNA double-strand breaks. Here, we applied the proximity ligation assay (PLA) to show that at such breaks WRAP53 accumulates in close proximity to H2AX and, furthermore as demonstrated by their co-im...
Methods We conducted further investigation of HCN2 interaction with γ-secretase using the proximity ligation assay (PLA). This assay can detect a protein-protein interaction when two proteins are closer than 40 nm. We performed PLA in several different materials; human embryonic kidney (HEK) cel...
The proximity ligation assay (PLA) has previously been used for the sensitive and specific detection of single proteins. In order to adapt PLA methods for the detection of cell surfaces, we have generated multivalent peptide–oligonucleotide–phycoerythrin conjugates ('burrs') that can bind adjacent ...
d Proximity ligation assay (PLA) was performed to identify interactions between EGFR and EphA2 (red) in grade I (n = 13), grade II (n = 14), and grade III/V (n = 13), and metastatic (n = 10) lung cancer tissues and their corresponding normal tissues (n =...
Proximity ligation assay(PLA)is a recently developed strategy for protein analysis in which a pair of proximity probes are designed to bind pairwise to target proteins via a DNA ligation reaction of oligonucleotides results in the formation of an amplifiable DNA strand suitable for analysis.It allo...
the proximity ligation assay (PLA). PLA used here utilizes species-specific secondary antibodies conjugated with distinct DNA primers. A hybridization step followed by circular DNA amplification with fluorescent probes to the conjugated DNA primers allows the visualization of proximity spots by ...
The proximity ligation assay (PLA) detects proteins via their interaction with pairs of proximity probes, which are antibodies coupled to noncomplementary DNA oligonucleotides. The binding of both proximity probes to their epitopes on the target protein brings the oligonucleotides together, allowing them...
Applying the proximity ligation assay (PLA) to mouse preimplantation embryos for identifying protein-protein interactions in situ. Analysis of protein-protein interactions in preimplantation embryos is hindered by the low cell number of the embryo. Here we describe the use of the proxi... I Bedzh...
These 12 HCIPs were subjected to the proximity ligation assay (PLA). None of the 12 HCIPs had been previously reported, but each interaction pair was successfully verified (Figure S2), which indicated that our assays reliably enriched for high-affinity and stable Rab interactions. However, like...