PLA技术全称为Proximity Ligation Assay,国内译为邻位连接或者邻近连接技术。PLA技术是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多克隆抗体的探针,即PLA probe(探针),识别目的蛋白,当这两个探针识别同一个蛋白时,两个探针之间的距离...
Henrik H. J. Persson为通讯作者在Proc Natl Acad Sci U S A期刊上发表题为“Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies”的文章[5],提出了一种高度特异的环状邻近连接试验(c-PLA),它在严格性、易用性和与低亲和力试剂的相容性方面优...
邻近连接分析,英文Proximity ligation assay,简称PLA, 中文翻译为邻位连接分析或者邻近连接分析技术。 邻近连接分析PLA是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。 邻近连接分析PLA由Fredriksson等人于2002年开发。到目前为止,它已成为一种成熟且普遍适用的免疫组化工具,用于高级和精确的蛋白质分析。
邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 发布邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 剧情介绍:
ProximityLigationAssayisatechnologythatextendsthecapabilitiesoftraditional immunofluorescenceandimmunohistochemistrytoincludedirectdetectionofproteins-protein interactionsandmodificationswithunparalleledspecificityandsensitivity.Targetproteinsare detectedwithsinglemoleculeresolution,allowingdigitalquantificationandinformationaboutthe ...
location in tissue samples offers exciting new opportunities in biomarker research. By providing the element of dual recognition to in situ analyses, pathology can expand into a new arena of analytes, beyond measuring the abundance of just a single protein.PLA™, a proximity ligation assay ...
Proximity Ligation Assay (PLA)简介 Proximity Ligation Assay (PLA)简介 1, 什么是PLA技术?PLA技术全称为Proximity Ligation Assay， 国内翻译为邻位连接或者邻近连接技术。PLA技术是一种特殊的免疫分析方法，可用于检测目标蛋白，蛋白相互作用等等。 该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多...
rnawhole-mountinsituhybridisationproximityligationassay40rish-pla41an 系统标签: rishhybridisationplarnaassayligation RESEARCHARTICLE RNAWhole-MountInsituHybridisation ProximityLigationAssay(rISH-PLA),anAssay forDetectingRNA-ProteinComplexesin IntactCells IoannisM.Roussis,MatthewGuille,FionaA.Myers*,GarryP.Scarlett...
ligation. Combining the specificity of enzyme-linked immunosorbent assay (ELISA) and sensitivity of polymerase chain reaction (PCR), PLA transforms the detection of protein into the detection of DNA nucleic acid sequence. Therefore, as an ultrasensitive protein assay, PLA has great potential for ...