Bedzhov, I. & Stemmler, M. P. Applying the proximity ligation assay (PLA) to mouse preimplantation embryos for identifying protein-protein interactions in situ. Methods Mol Biol 1233, 57-64 (2015).Bedzhov I, Stemmler MP. 2015. Applying the Proximity Ligation Assay (PLA) to mouse ...
Here, we applied the proximity ligation assay (PLA) to show that at such breaks WRAP53 accumulates in close proximity to H2AX and, furthermore as demonstrated by their co-immunoprecipitation (IP) binds to H2AX, in a manner dependent on the ATM and ATR kinases. Moreover, formation of ...
Proximity ligation assay Monoclonal antibody Diagnostics Clostridium qPCR dPCR 1. Introduction The proximity ligation assay (PLA) [1] combines the specificity of antibody-based detection of proteins with the exquisite sensitivity and dynamic range of real time quantitative PCR (qPCR) to detect antigens...
3h)37. The proximity ligation assay (PLA) also suggested that Rab7 forms a complex with Dl in vivo (Fig. 3g). Fig. 3: Delta and Notch differentially colocalize with Rab7 and Rab4. a, b Dl (magenta) is colocalized with Rab7-EYFP (green in a) and Rab4-EYFP (green in b) at ...
To this end, we used the proximity ligation assay (PLA). As a positive control, we used two different primary antibodies against Prx2 to demonstrate its well-established self-assembly into homo-oligomers (Fig. 1c, upper left panel). The in situ co-proximation of Prx2 and STAT3 was ...
By using an in situ proximity ligation assay (PLA),35 we confirmed that DSS increased MAM in the colon (Figure 4H). VBIT-12 prevents DSS-induced inflammation and increased serum mtDNA levels The inflammatory response is a central pathological process in IBD,36 and a key mediator of ...
(Vazyme, Nanjing, China), then these fragments were end-repaired, dA-tailed, and adaptor ligated. ligation products were PCR-amplified and obtained a DNA library. The concentration of the DNA library was quantified using a Qubit dsDNA HS Assay Kit. Hybridization was performed using the HPV ...
4.9. Proximity Ligation Assay (PLA) For each well, 40,000 HeLa cells were seeded in 24-well plates, grown for 48 h, fixed with 4% paraformaldehyde and permeabilized with 0.05% (v/v) Triton X-100. PLA assays using the Duolink kit (Sigma Aldrich, St. Louis, MO, USA, DUO 9200) wer...
The cytolethal distending toxin (CDT) is produced by many pathogenic Gram-negative bacteria and is considered as a virulence factor. In human cells, CDT exposure leads to a unique cytotoxicity associated with a characteristic cell distension and induces
The "in situ" proximity ligation assay to probe protein-protein interactions in intact tissues. Methods Mol Biol. 2014;1174:397–405. Article CAS Google Scholar Alam MS. Proximity ligation assay (PLA). Curr Protoc Immunol. 2018 Sep;20:e58. Article Google Scholar Azzimonti B, Dell'oste ...