PLA技术 全称为Proximity Ligation Assay 是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多克隆抗体的探针,即PLA probe(探针),识别目的蛋白,当这两个探针识别同一个蛋白时,两个探针之间的距离靠近,产生了所谓的邻近效应(proximity)。
邻近连接分析,英文Proximity ligation assay,简称PLA, 中文翻译为邻位连接分析或者邻近连接分析技术。 邻近连接分析PLA是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。 邻近连接分析PLA由Fredriksson等人于2002年开发。到目前为止,它已成为一种成熟且普遍适用的免疫组化工具,用于高级和精确的蛋白质分析。
PLA技术全称为Proximity Ligation Assay， 国内翻译为邻位连接或者邻近连接技术。PLA技术是一种特殊的免疫分析方法，可用于检测目标蛋白，蛋白相互作用等等。 该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多克隆抗体（）的探针，即PLA probe（PLA探针），识别目的蛋白，当这两个探针识别同一个蛋白时...
邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 发布邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 剧情介绍:
Representative image of Proximity Ligation Analysis (PLA) of protein-protein interactions between Caspase 6 (CASP6) and Caspase 3 (CASP3). HeLa cells were stained with anti-CASP6 rabbit purified polyclonal antibody 1:1200 and anti-CASP3 mouse monoclonal ...
measuring the abundance of just a single protein.PLA™, a proximity ligation assay technology, is capable of detecting single protein events such as protein interactions (e.g. protein dimerization) and modifications (e.g. protein phosphorylation).The Duolink® reagent series, based on PLA, ...
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Proximity ligation assay(PLA) is a recently developed strategy for protein analysis in which a pair of proximity probes are designed to bind target proteins via a DNA ligation reaction of oligonucleotides resulting in the formation of an amplifiable DNA strand suitable for real-time PCR.Because of...
α-Synuclein proximity ligation assay (PLA) has proved a sensitive technique for detection of non-Lewy body α-synuclein aggregate pathology. Here, we describe the MJF-14 PLA, a new PLA towards aggregated α-synuclein with unprecedented specificity, usin