Simon Fredriksson以第一作者在Nat Biotechnol期刊上发表题为“Protein detection using proximity-dependent DNA ligation assays”的文章[1],改文章主要介绍了提出了通过两个DNA适配子与目标蛋白的结合,促进寡核苷酸连接到每个适配子亲和探针,两个这样的近端探针连接产生一个可扩增的DNA序列,可扩增DNA序列的数量反映目标...
Simon Fredriksson以第一作者在Nat Biotechnol期刊上发表题为“Protein detection using proximity-dependent DNA ligation assays”的文章[1],改文章主要介绍了提出了通过两个DNA适配子与目标蛋白的结合,促进寡核苷酸连接到每个适配子亲和探针,两个这样的近端探针连接产生一个可扩增的DNA序列,可扩增DNA序列的数量反映目标...
Simon Fredriksson以第一作者在Nat Biotechnol期刊上发表题为“Protein detection using proximity-dependent DNA ligation assays”的文章[1],改文章主要介绍了提出了通过两个DNA适配子与目标蛋白的结合,促进寡核苷酸连接到每个适配子亲和探针,两个这样...
★ Navinci 是一家瑞典生物技术公司,是邻近连接技术 (Proximity Ligation Assay,PLA)的先驱,专门开发用于研究蛋白质相互作用的创新解决方案。 ★ 2020年Navinci公司将Proximity Ligation Assay(PLA)技术进行了更新,命名为:Naveni® Proximity Ligation Technology,此技术基于一项新的UnFold专有技术,与传统PLA技术相比,实...
Simon Fredriksson以第一作者在Nat Biotechnol期刊上发表题为“Protein detection using proximity-dependent DNA ligation assays”的文章[1],改文章主要介绍了提出了通过两个DNA适配子与目标蛋白的结合,促进寡核苷酸连接到每个适配子亲和探针,两个这样的近端探针连接产生一个可扩增的DNA序列,可扩增DNA序列的数量反映目标...
Protein detection using proximity-dependent DNA ligation assays, Nature Biotechnology 20, 473 - 477 (2002) 2) Solid phase PLA (固相PLA) 在液相PLA的基础上引入了一个捕获抗体,该捕获抗体被固定在固相载体上(磁珠、微孔板等),当目标蛋白被捕获后,通过Proximity Probe进行识别和定量。Detection of ...
in situ assaysprotein‐protein interactionProximity ligation assay (PLA), also referred to as Duolink? PLA technology, permits detection of protein‐protein interactions in situ (at distances <40nm) at endogenous protein levels. It exploits specific antibodies identifying (either directly or indirectly)...
irus-Infected Cells Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected CellsProximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected CellsColocalization...
such as BRET, FRET and BiFC act on genetically modified cells with usually overexpressed fusion protein complexes.Dual recognitionIn situ PLA technology requires positive identification of two different epitopes on the same protein or complex, resulting in profoundly enhanced specificity over assays that ...
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