Protein interactionReceptor tyrosine kinaseSignal transductionImagingSpatiotemporal aspects of protein-tyrosine phosphatase (PTP) activity and interaction partners for many PTPs are elusive. We describe here an elegant and relatively simple method, in situ proximity ligation assay (in situ PLA), which ...
in situ proximity ligation assay (PLAprotein‐protein interaction (PPIsignaling pathwayUnderstanding signaling pathway networks via protein-protein interactions (PPIs) at the cellular level is a significant task that has not yet been completed. Here, a systems approach that computationally infers ...
In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sen...
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Follow the Duolink® In Situ Brightfield Protocol to use this product.Visi...
Olczak, M. Olczak, In situ proximity ligation assay (PLA) analysis of protein complexes formed between Golgi-resident, glycosyl- ation-related transporters and transferases in adherent mammalian cell cultures, in: W.J. Brown (Ed.) Methods Mol Biol, Springer New York 2016, pp. 133-143....
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Use theDuolink®In Situ Fluorescence Protocolfor this product. A set ofsho...
PLA is a registered trademark of sigma-aldrich Co. LLC 基本信息 NACRESNA.32 产品性质 质量水平200 产品线Duolink® technique(s)proximity ligation assay: suitable 荧光λex360 nm; λem460 nm 适用性suitable for fluorescence-detection automated sequencing ...
Step 4: Proximity ligation assay (Duolink kit) Dry slides well and re-draw circles with hydrophobic pen Blockingw/ provided solution. 45 min RT Primary antibodies:H3K4dime (1/100) + biotin (1/100) in antibody dilution solution, 1mL usually good for whole exp. Ovenight 4°C. ...
rna whole-mount in situ hybridisation proximity ligation assay (rish-pla), an assay for detecting rna-protein complexes .包埋rna原位杂交接近结扎试验(rish-pla),检测完整 文档格式: .pdf 文档大小: 524.33K 文档页数: 13页 顶/踩数: 0/0
proximity ligation assay: suitable suitability【适用性】 suitable for brightfield suitable for fluorescence shipped in【运输】 wet ice storage temp.【储存温度】 2-8℃ 安全信息 Storage Class Code【储存分类代码】 10 - Combustible liquids WGK