邻近连接分析,英文Proximity ligation assay,简称PLA, 中文翻译为邻位连接分析或者邻近连接分析技术。 邻近连接分析PLA是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。 邻近连接分析PLA由Fredriksson等人于2002年开发。到目前为止,它已成为一种成熟且普遍适用的免疫组化工具,用于高级和精确的蛋白质分析。
Proximity ligation assay (PLA), also referred to as Duolink? PLA technology, permits detection of protein‐protein interactions in situ (at distances <40nm) at endogenous protein levels. It exploits specific antibodies identifying (either directly or indirectly) the two proteins of interest and ...
Proximity Ligation Assay (PLA)简介 Proximity Ligation Assay (PLA)简介 1, 什么是PLA技术?PLA技术全称为Proximity Ligation Assay， 国内翻译为邻位连接或者邻近连接技术。PLA技术是一种特殊的免疫分析方法，可用于检测目标蛋白，蛋白相互作用等等。 该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多...
之后利用邻位连接(Proximity ligation assay,PLA)Duolink技术证明,在膀胱癌细胞株5637细胞中,DHCR24与HRAS存在直接的相互作用,且这种相互作用发生于细胞核外;进一步的... 毛竞 - 《辽宁大学》 被引量: 0发表: 2020年 加载更多来源期刊 BIO-PROTOCOL 2016/01/01 研究点推荐 Duolink Proximity Ligation Assay (...
全称为Proximity Ligation Assay 是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多克隆抗体的探针,即PLA probe(探针),识别目的蛋白,当这两个探针识别同一个蛋白时,两个探针之间的距离靠近,产生了所谓的邻近效应(proximity)。
About this protocol Cite this protocol Bretteville, A., Demiautte, F., Chapuis, J. (2017). Proximity Ligation Assay: A Tool to Study Endogenous Interactions Between Tau and Its Neuronal Partners. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press...
Proximity ligation assay (PLA) is a newly developed technique that outperforms the traditional immunoassays for visualizing the in situ endogenous protein-protein interactions and localizations and the activation of proteins in cell culture systems as well as in tissue sections. PLA, when combined with...
In Situ Hybridization-Proximity Ligation Assay, ISH-PLA This protocol is available for download on Owens's lab page:https://www.cvrc.virginia.edu/Owens/files/ISH-PLA%20Owens%20lab%20protocol.docx. And this is a published protocol on Nature Methods:https://www.nature.com/articles/nmeth.2332...
aproximityligationassaytechnology,iscapableofdetectingsingleproteineventssuchasproteininteractions(e.g.proteindimerization)andmodifications(e.g.proteinphosphorylation).TheDuolink®reagentseries,basedonPLA,offersanunprecedentedlevelofsensitivityandspecificityforproteindetectioninfixedcellsandtissues.Theprincipleofthe...