Recently developed proximity-dependent biotin identification (BioID) has been actively investigated as an alternative PPI mapping method because of its usefulness in uncovering transient PPI. Here, as an example of proximity labeling proteomics application in the testis, we generated two transgenic mouse...
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J. Cell Biol. 196, 801–810 (2012). Article CAS PubMed PubMed Central Google Scholar Gupta, G. D. et al. A dynamic protein interaction landscape of the human centrosome-cilium interface...
Proximity-dependent labeling has proven to be a valuable method for revealing protein-protein interaction networks in living cells. A mutant form of the biotin protein ligase enzyme from Aquifex aeolicus (BioID2) underpins this methodology by producing biotin that is attached to proteins that enter ...
International Journal of Molecular Sciences Review Mapping the SARS-CoV-2–Host Protein–Protein Interactome by Affinity Purification Mass Spectrometry and Proximity-Dependent Biotin Labeling: A Rational and Straightforward Route to Discover Host-Directed Anti-SARS-CoV-2 Therapeutics Rosa Terracciano 1,*,...
Here, we applied the BioID proximity-based biotin labeling technique [39,40] to identify interacting proteins of eIF3A in two human cell lines: the human colorectal carcinoma cell line HCT-116 and the human embryonic kidney cell line HEK-293T. These cell lines differed slightly in the ratio...
As a demonstration of the spatial specificity and depth of coverage in mammalian cells, we applied APEX2 labeling with biotin‐aniline (Btn‐An) in the mitochondrial matrix, capturing all 13 mitochondrial messenger RNAs and none of the cytoplasmic RNAs. APEX2‐mediated Btn‐An labeling of RNA ...
BiotinFluorescent LabelingDissociation RateProximity-Dependent BiotinylationBecause streptavidin is a homotetramer, it can bind multiple biotinylated ligands and cause target aggregation. To allow biotin detection without clustering, we previously engineered monomeric streptavidin (mSA) that is structurally similar...
Protein crosslinking and BioID (proximity-dependent biotin identification) permit unbiased screening of protein proximity for a protein of interest. Here, we evaluate these approaches and their applications in living eukaryotic cells.doi:10.1007/s00018-013-1287-3...
Proximity-dependent labeling has proven to be a valuable method for revealing protein-protein interaction networks in living cells. A mutant form of the biotin protein ligase enzyme from Aquifex aeolicus (BioID2) underpins this methodology by producing biotin that is attached to proteins that enter ...
Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID Proximity-dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a prot... R Varnaitė,SA Macneill - 《Proteomics》...