Interleukin-1α is a suggested dual-function cytokine that diverged from interleukin-1β in mammals potentially by acquiring additional biological roles that relate to highly conserved regions in the pro-domain of interleukin-1α, including a nuclear localisation sequence and histone acetyltransferase-bind...
We found that overexpression of the identified papain-like cysteine protease resulted in an improvement in the yield of cyclic kB1, while this was not the case for the overexpression of protein disulfide isomerases, despite their interaction being verified using bimolecular flu...
Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein–protein interactions. Although the conventional approach of enriching biotinylated proteins is widely implemen
To further characterize ANXA3 function in HCV, we performed proximity labeling using ANXA3-BioID2 as bait in HCV-infected cells. Two of the top proteins identified proximal to ANXA3 during HCV infection were the La-related protein 1 (LARP1) and the ADP ribosylation factor-like protein 8B (...
Next, the entry process of the SARS-CoV-2 pseudovirus was attenuated by an ITGB1 antibody in a dose-dependent manner (Figure 4F). Moreover, we knocked down the expression of ITGB1 in HeLa-hACE2 cells by RNAi assay (Figure 4G). As expected, ITGB1 expression was significantly reduced by...
, we employed the proximity-dependent biotin identification (BioID) approach that allows for the labeling of proximal proteins in living cells and their purification under harsh denaturing conditions [12, 80], to determine the interactome of detergent-insoluble poly-GR, -PR, and -GA aggregates. ...
Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling Article Open access 20 August 2022 OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools Article Open access 29 February 2024 Spatially resolved mapping of ...
Because many cancer types are dependent on importin beta-1 expression for their proliferation and survival15–20, importin beta-1 has been proposed as a novel therapeutic target21 and inhibitors are being devel- oped, e.g. Importazole22 and INI-4323. Interactomic analyses of importin-1 beta ...
APEX2, an engineered ascorbate peroxidase for high activity, is a powerful tool for proximity labeling applications. Thanks to its lack of disulfides and the calcium﹊ndependent activity, APEX2 can be applied intracellularly for targeted electron microscopy imaging or interactome mapping when fusing ...
(1996). Structure and function in rhodopsin - cysteines 65 and 316 are in proximity in a rhodopsin mutant as indicated by disulfide formation and interactions between attached spin labels. Biochemistry 35, 14040 -14046.Yang K. , Farrens D. L. , Altenbach C. , Farahbakhsh Z. T. , ...