This calculator takes a mass* of nucleotides* and strand length. It returns the total number of copies of the strands present in that amount of material. Concentration* depends on your sample. if you have DNA* at a concentration of 100 ng/ul. Then putting 100 in for mass along with th...
BLAST Search Check for oligonucleotide sequence homology Reaction Conditions Definitions and values used in the OligoEvaluator™ calculator ParameterValue Oligo Concentration The default value = 250.0 nM. Monovalent Ion Concentration This is the concentration of all monovalent ions, e.g...
Firstly, the melting temperature obtained by PrimerMapper was compared with the NEB calculator for NEB Taq DNA polymerase (Fig. 7c; r2 = 0.979). The NEB calculator ® ®uses the algorithm defined by SantaLucia28 and is salt corrected as described by Owczarzy et al.29 for ...
Impact of concentration of universal primer on singlet PCR.Wentao XuZhifang ZhaiKunlun HuangNan ZhangYanfang YuanYing ShangYunbo Luo
Final concentrationBuffer w/MgCldNTP mixExTaqHO
the purity of the DNA (absorbance 260/280 nm ratio) was determined by using a NanoDrop®156 ND-1000 UV-Vis spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and the yield concentration was quantified by using the Quant-iTTM PicoGreenTM dsDNA Assay Kit (Thermo Fisher Sc...