Initial generation of the sequen- cing libraries utilised a one-step PCR with a total of 30 cycles, a mixture of Hot Start and HotStar High Fidelity Taq polymerases (Qiagen). The primers used were 939F (TTGACGGGGGCCCGCAC) and 1492R (TACCTTGTTACGACTT) to sequence the V6–V9 regions of...
Q-PCR was processed to obtain a more accurate library concentration. At last, six libraries were sequenced on an Illumina NovaSeq platform to generate 150 bp paired-end reads, according to the manufacturer’s instructions (NCBI BioProject accession number: PRJNA1056490). The cDNA library ...
Single-stranded cDNA was prepared from 1 μg of total RNA using reverse transcriptase with oligo-dT primer according the manufacturer’s instructions (Promega, USA). Two microlitres of each cDNA was subjected to PCR amplification using specific primers for CD47, mitf, RANK, Src, c-fos, NF...
WP3 in response to changing salinity was analyzed by RNA fingerprinting using arbitrarily primed PCR (RAP-PCR). Ninety primer sets were used to scan two different RNA pools derived from cultures of 1% and 7% NaCl concentrations. Forty-three putative differential-expressed fragments were identified,...
The concentration of all TGF-β used in this part of the experiment was 5 ng/ml Full size image We showed that TGF-β could promote the nuclear translocation of HIF-1α, p-Smad2, and p-Smad3 by immunofluorescence assay and western blotting; this translocation was blocked by knockdown of ...
However, dPCR faces hurdles like sample size constraints, false positives arising from PCR errors, and the absence of standardized sample processing protocols. The influence of DNA purity and concentration on dPCR results warrants protocol optimization, and there are also difficulties in separating ...
The outcomes were similar between the Real-time PCR analysis and the transcriptome sequencing analysis, except the relative abundance of CCL4. 4 Discussion Calcium is an essential mineral, which must be absorbed from the diet. Similar to mammals, to reach the required Ca concentration for ...
The concentration of 27 different transition, post-transition, rare, and radioactive metals were determined in molten snow and frost flower, as well as filtered samples. The range of three highest detected metal concentrations among samples were: Hg (3.294–134.485 μg/L), Fe (0.719–...
The PCR primers were designed from the human ER-α-36 cDNA sequence in NCBI. Cell culture, transfection, the induction of an MCF-7 stable cell line, and Hec1A cell prolifer- ation assay. MCF-7, Hec1A and HEK-293 cells were grown according to the protocols recommended by ATCC....
underlying sediment consisted of light grey silt and showed a considerable decrease in concentration of the latter porewater components salinity (P < 0.05) from and an 56 g/kg increasing in the mat StoO8422− /gC/kl−graatti1o0. Tcmhebdsrfo(pFiogc. c2u, rSruepdpalle...