(wild-type) enzyme gene alleles Usual doses may lead to sub-therapeutic drug concentration and possible non-response EM: Extensive Metabolizer 2 copies of active (wild-type) enzyme gene alleles Usual doses lead to expected drug concentrations and response IM: Intermediate Metabolizer 1 inactive ...
We performed qPCR using SYBR green in an Applied Biosystems 7900HT Fast Real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) and the standard protocol. A serial dilution of cDNA was used to generate a standard curve for each primer set, and this curve was used to calculate ...
. Each amplification was performed in a total volume of 20 μl containing 5 μl QuantiTect SYBR Green PCR kit (Qiagen) and the specific primer mix (PrimerAssay—Qiagen). PCR conditions were run according to the manufacturer’s manual (Qiagen). A melting point analysis was conducted for...
SARS-CoV-2 PCR detection was carried out using Primerdesign's (Eastleigh, UK) Coronavirus COVID-19 Genesig RT-PCR assay (Z-Path-COVID-19-CE) and a Roche LightCycler 480 in a 96-well format. All kits were used according to manufacturer's guidelines. SARS-CoV-2 Serology Assays Two ...
We performed qPCR using SYBR green in an Applied Biosystems 7900HT Fast Real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) and the standard protocol. A serial dilution of cDNA was used to generate a standard curve for each primer set, and this curve was used to calculate ge...
PCR was performed using an MJ Mini Gradient Thermal Cycler (Bio-Rad, Hercules, CA, USA). Universal primers ITS1 and ITS4 were used for the amplification of the internal transcribed spacer regions (ITS1/ITS2) [29]. The amplification of actin and calmodulin gene fragments was performed with...