10% of the standard primer concentration used for the longer sequencer-specific indexing primers. The rationale was the short primers would initiate PCR more efficiently in early cycles and increase the concentration of the 16S rRNA target sequences for the longer modified primers sufficiently to o...
Antioxidant capacity of ST11 hv-CRKP C1789 were assessed byaABTS andbSOD activities determination assays. Relative scavenging rates was the calculated clearance per concentration unit. The SOD activity assay was performed with six biological replicates. *p = 0.021, **p = 0.010, ****p ...
Although exercise altered the concentrations of other SCFAs, such as propionic acid, in young adult mice with surgery, valeric acid was the only SCFA whose concentration in the blood was decreased by exercise in old mice with surgery. In supporting the role of valeric acid in the exercise ...
Quantitative RT-PCRs were carried out using QuantiTect® SYBR® Green PCR Kit (Qiagen) with specific primers (Supplementary Material), in a Rotor-Gene 6000 analyzer (Corbett). Beta-Actin encoding gene Actb was used as house-keeping gene. The relative mRNA amount in each sample was calcula...
First-strand cDNA was synthesized with random primers using High Capacity cDNA Reverse Transcription Kit (Takara, Dalian, China). qPCR was performed using Power SYBR Green (Takara, Dalian, China) on a CFX96 Real-Time PCR Detection System (Bio-Rad, USA). Data was collected and normalized to...
Moreover, an increase in tissue concentration of ATP in the RVLM leads to an increase in activity of the sympathetic premotor neurons, resulting in the increased central sympathetic drive [37]. Expression of the mitochondrial electron transport chain enzymes is essential for generation of ATP. Our...
production (Fig.4C). Notably, the pyrophosphate-to-phosphate ratio (Fig.4D) resulting from ATP hydrolysis was significantly lower (6.1-fold) in ASMCs incubated with a high glucose concentration (0.12 ± 0.01) than in those incubated with a control glucose concentration (0.75 ± ...
RNA extracted from the cells by using Trizol reagent (Applied Biosystem) was retrotranscribed into cDNA using the Multiscribe MuLV retrotranscriptase and random primers; cDNA was amplified by Real-time qPCR using the Power SYBR Green PCR Master Mix, according to the manufacturer's protocol (Applie...
Absorbency of the DNA solution was measured at A260 with an ultraviolet–visible spectro- photometer (UV-2450(PC)S, Shimadzu, Kyoto, Japan) to determine the DNA concentration. Final extractions were stored at À20 1C until used for PCR amplification. Approximately 50 ng of DNA extract was...
Deep-sequencing libraries were generated by PCR. TruSeq HT Dual Index primers were used to label each sample (Supplementary Table 6). Pooled libraries were subjected to paired-end sequencing using MiniSeq (Illumina). iPS cell genome editing iPSC generation has been described previously39. Briefly...