An improved method for performing a nested polymerase chain reaction (PCR) amplification of a targeted piece of DNA, wherein by controlling the annealing times and concentration of both the outer and the inner set of primers according to the method disclosed, highly specific and efficient ...
proliferationin vitro, while a high concentration (5-10 mmol/L) could inhibit cell proliferation (P<0.05). According to the literature and the experimental data, 0.1 mmol/L NaF was selected as the following experimental concentration. The levels of alizarin red S staining were increased after Na...
The cucumber Ubiquitin extension protein (UBI-ep) was used as an internal control, and relative amounts of mRNA were calculated using the comparative threshold cycle method. Four biological and three technical replicates were performed for each gene. Specific primers used for qRT-PCR were listed ...
synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling.Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers)....
Conversely, in rhizosphere and non-rhizosphere soils, the community composition of streptomycetes was affected strongly even by modest compost amendment. The application of a streptomycetes-specific PCR primer set combined with DGGE analysis provided a rapid means of examining the distribution and ecology...
PCR reactions were performed and analyzed on LightCycler 480 Instrument (Roche, Indianapolis, IN). PCR primers were shown as follows: 5′-TCTCCAAAGGAGGTTACCTGC-3′, 5′-AGACTCCCCTCAGTAAAGTGAC-3′ (xCT); 5′-CGGAACCGCTCATTGCC-3′, 5′-ACCCACACTGTGCCCATCTA-3′ (β-actin). Relative ...
aReevaluation of the specificity of previously published PCR amplification primers and group specific [translate] a他是我们班上最高的学生 He is in our class the highest student [translate] a每个真正的奢侈品牌都曾历经时间沉淀,培养了一批忠实的追随者,而其背后也总会有一个品牌故事,或引人入胜,或...
Individual mosquitoes were identified to species-level using species-specific PCR primers forAn. gambiae s.s.andAn. arabiensis(Table3)63. Each 20 μl reaction contained 20–40 ng of gDNA, 10 μl HotStart Taq 2X Master Mix (New England Biolabs, UK) and 25 pmol/ml of primers AR-3T, GA...
The hypervariable region V3–V4 of the bacterial 16S rRNA gene was amplified with primer pairs 338F: 5'- ACTCCTACGGGAGGCAGCA-3' and 806R: 5'- GGACTACHVGGGTWTCTAAT-3'. PCR products were checked on agarose gel and purified through the Omega DNA purification kit (Omega Inc., Norcross, ...
The primers for mucin 2, Zonula occludens-1 (ZO-1), Occludin, Claudin1, and β-actin were designed using Primer 3 and were shown in Table 3. Data are presented in arbitrary units as relative mRNA levels normalized to endogenous gene β-actin according to the 2−ΔΔCt method (Livak...