Samples for subsequent analysis had an RNA integrity number > 8.0, and a portion of each was diluted to 100 ng/μL with RNase-free water before cDNA synthesis. One hundred nanograms of RNA was used for cDNA synthesis, and the quantitative PCR was performed as described previously. Primers ...
The bottom layer of the soft agar plate was made with 0.6% agarose, poured in a six multi-well dish and allowed to settle for one hour at room temperature. The harvested cells were mixed with 0.3% agarose at a concentration of 8,000 cells/well and were overlaid above the bottom agar....
Initially, a DNA fragment coding for a full-length human CD24 fragment was amplified by PCR using the plasmid pCMV-SPORT6-CD24 as a template using primers Kozak-HindIII-CD24-F (5′-CTGGAAGCTTGCCACCATGGATGGGCAGAGCAATGGTGGC-3′) and XbaI-CD24-R (5′-TCATCTAGAGTATTAAGAGTAGAGATGCAGA...
in stone fruit trees combining a set of universal primers with the inclusion of an internal control for the diagnosis of Agriculture 2018, 8, 125 12 of 34 brown rot caused by the three more important species. More recently, the RT-PCR method developed by Brouwershaven et al. [88] was ...