post‐translationally modified proteinsprotein phosphorylationdisulfide bond detection and mappingEven though most medicines have historically been small molecules, many newly approved drugs over the last two decades have been derived from proteins. For the past few years, protein therapeutics have been ...
In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.Agnew, BrianGee, Kyle RNyberg, Tamara GAgnew B, Gee KR, Nyberg TG (2007) Labeling and Detection of Post Translationally Modified Proteins. US Patent 2007/0249014 A1....
Post-translationally modified proteinsare crucial for a wide variety of cellular events, fromcell signalingtoDNA replication. However, recent studies have suggested that there are times when the presence or absence of post-translational alterations in self-proteins can profoundly affectantigen recognitionin...
Numerous studies of native proteins have been reported on protein folding in this half century. Recently, post-translationally modified proteins are also focused on protein folding. However, it is still difficult to prepare such types of proteins because it requires not only the chemical but also ...
Disclosed herein are modified phage comprising a fusion protein located on the surface of the phage wherein the fusion protein comprises a surface protein and a post-translationally modified protein. Also disclosed are methods of making and using modified phage comprising post-translationally modified p...
Having shown that, for samples containing only purified proteins, large portions of the protein sequence can be recovered by combining multiple MS/MS spectra from overlapping peptides, we now extend the Shotgun Protein Sequencing approach to the analysis of mixtures of modified proteins. A mixture ...
Ribosomally synthesised and post-translationally modified peptides (RiPPs) are a class of structurally complex NP with diverse bioactivities. RiPPs have recently been shown to occupy a much larger expanse of genomic and chemical space than previously appreciated, indicating that annotation of RiPP BGCs ...
In particular, this work emphasizes the selective recognition of posttranslational modifications of histone proteins in the context of peptides through differential non-covalent interactions. DCC was used to identify and synthesize small molecule receptors, in the form of amphiphilic macrocycles with ...
almost all the proteins detected in this study were modified. Moreover, these are cleavage maturation events where no evidence was found of the unmodified protein. Although various modification types were detected, the predominant PTM observed here was proteolysis. Beyond simply showing expression of ...
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a class of cyclic or linear peptidic natural products with remarkable structural and functional diversity. Recent advances in genomics and synthetic biology, are facilitating us to discover a large number of new ribosomal natur...