Identification of the post-translationally modified proteins not only enabled the biomarker prediction associated with various disease conditions but also has a great impact in target based drug discovery process. Mass spectrometry based proteome profiling aided the identification and characterization of these...
provides invaluable insight into the cellular functions underlying etiological processes. Technically, the main challenges to studying post-translationally modified proteins are the development of specific detection and purification method...
According to previous research, 70% of the total protein in the human body is post-translationally modified by glycosylation [10], and 1% of the human genome is involved in the synthesis and modification of glycoprotein [11]. Notch plays an important role in the development of animal embryonic...
while the reverse process is removal of the corresponding phosphate group by protein phosphatase.BGlycosylation, Glycosylation is the process of adding sugars to proteins or lipids under the control of enzymes, starting
In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.Agnew, BrianGee, Kyle RNyberg, Tamara GAgnew B, Gee KR, Nyberg TG (2007) Labeling and Detection of Post Translationally Modified Proteins. US Patent 2007/0249014 A1....
Due to the unique nature of spermatozoa, which are transcriptionally and translationally silent, the regulation of capacitation is based on the formation of posttranslational modifications of proteins (PTMs). However, the interactions between different t
making their analysis and identification particularly difficult. During the past two decades, chemical methods have significantly contributed to the identification and analysis ofposttranslationally modified proteins. In general, these techniques can be broadly classified into two categories, which both ...
A variety of proteins can be modified by KATs, including histones, transcription factors and nuclear import factors, to regulate numerous diverse cellular events involving DNA recognition, protein stability and protein–protein interaction [138]. Acetylation is reversible and the acetyl groups can be ...
The invention provides methods and compositions for the rapid and sensitive detection of post-translationally modified proteins, and particularly of those with post-translational glycosylations. The methods can be used to detect O-GlcNAc posttranslational modifications on proteins on which such ...
‘non-self’ antigen. The number of examples in whichposttranslationally modified proteinstrigger B- or T-cell responses has increased sharply during the last 5 years (see Ref.[1]for an overview). The modifications associated with autoimmunity are generally quite specific as far as T-cell ...