Pathways related to PMA- differentiated THP1 human monocytic leukemia cells revealed by RNA- Seq. Sci China Life Sci 2015; 58(12): 1282-1287.Zeng C, Wang W, Yu X, Yang L, Chen S and Li Y: Pathways related to PMA‑differentiated THP1 human monocytic leukemia cells ...
Phosphorylated glucosamine inhibits the inflammatory response in LPS-stimulated PMA- differentiated THP-1 cells[J]. Carbohydr Res, 2010, 345(13): 1851-5.Phosphorylated glucosamine inhibits the inflammatory response in LPS-stimulated PMA-differentiated THP-1 cells[J] . Jung-Ae Kim,Chang-Suk Kong,...
In conclusion,THP-1 cells could be differentiated into macrophages with PMA at concentrations of 1-100 ng/ml, and increasinginduction concentration could increased inflammatory factors secretion including TNF-α and IL-6, but had nosignificant effects on expression and distribution of membrane surface ...
参考文献:Tedesco S, et al. (2018) Convenience versus Biological Significance: Are PMA-Differentiated THP-1 Cells a Reliable Substitute for Blood-Derived Macrophages When Studying in Vitro Polarization?. Front. Pharmacol. 9:71. doi: 10.3389/fphar.2018.00071©...
Anti-inflammatory Activity of the Oriental Herb Medicine, Arisaema cum Bile, in LPS-Induced PMA-Differentiated THP-1 Cells: Immunopharmacology and Immunoto... Anti-inflammatory activity of the oriental herb medicine, Arisaema cum Bile, in LPS-induced PMA-differentiated THP-1 cells. Immunopharmacology...
虽然是目前比较通用的巨噬细胞模型,但既往文献关于PMA诱导THP-1模型中,不同PMA诱导条件对细胞表面标志物CD11b、CD14表达等尚无系统的比较研究。基于此,本研究拟对不同浓度PMA作用THP-1细胞后,其CD11b、CD14的表达进行分析,初步考察了PMA诱导THP-1细胞分化的条件,为后续相关实验提供实验依据。 1 材料与方法 1.1 ...
The inhibitory rate of the secretion of proMMP-9, the activated MMP-9 and proMMP-2 in the differentiated THP-1 cells was 52.90%, 53.79% and 47.80%, respectively. The inhibitory rate of invasive potential in the undifferentiated cells and the differentiated THP-1 cells was 41.82 % and ...
【摘要】目的 用流式细胞术明确单独使用PMA诱导THP-1细胞分化而来的巨噬细胞的分化情况.方法首先用PMA刺激THP-1细胞分化为巨噬细胞,再分别使用LPS、IFN-γ和IL-6将细胞诱导为M1型巨噬细胞,用IL-4、IL-13和IL-6将细胞诱导为M2型巨噬细胞.显微镜下观察三种细胞的形态学改变,并用流式细胞术检测各细胞主要CD分子...
1巨噬细胞M1和 M2亚型相关基因表达的影响 * 章述军 雷青松 李 麟 秦 波 黄文祥 (重庆医科大学附属第一医院感染科, 重庆市传染病与寄生虫病重点实验室, 重庆400016) 摘要 佛波酯诱导THP-1单核细胞系分化为巨噬细胞的模型广泛被使用, 目前主要使用高、 低浓度两种方案, 其是否对M 1和M2亚型相关基因的表达有...
The inhibitory rate of the secretion of proMMP-9, the activated MMP-9 and proMMP-2 in the differentiated THP-1 cells was 52.90%, 53.79% and 47.80%, respectively. The inhibitory rate of invasive potential in the undifferentiated cells and the differentiated THP-1 cells was 41.82 % and ...