Antoine Fattaccioli, etc. M1 and M2 macrophages derived from THP-1 cells differentially modulate th...
（3）接种细胞：收集THP-1细胞并进行计数，用诱导培养基重悬细胞沉淀调整细胞密度为5*10^5/mL，将细胞...
THP-1是人单核细胞，经过PMA刺激分化为巨噬细胞。实验中常以0.1ug/ml 的PMA处理THP-1 72h 使其成为巨噬细胞。
Phorbol 12-myristate 13-acetate (PMA) is commonly used to activate protein kinase C (PKC) and a Standard Reagent for THP1 cell differentiation into macrophages-type cells.
至于M1和M2的实验,我暂时还没经验,但可以参考一些文献,比如《Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA》和《Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFN-γ + LPS), M(IL-4) and M(IL-10) ...
(PMA)ofinvitroculturedpromonocytesTHP1.MethodsTHP1cellswerefirsttreatedwithPMAgradientdoses(0,25,50,100,200,400ng/ml)for24h,andthecellviabilitywasmeasuredbyCCK8assay.TheninvitroTHP1cellswerestimulatedby0,10,50ng/mlPMArespectivelyfor24hand48h.FlowcytometrywasusedtodetectcellsurfacemarkersCD11bandCD14....
The concentration of 80ng/ml of PMA, the induction time of 24h, and the cell seeding density of 5脳105 cells/ml, could respectively facilitates a relatively higher CD14 positive rate in THP-1 cells. Under this optimized conditions, the CD14 positive rate of TH...
Isotype control;“-” CD16,CD80 and CD86;A:The expression of CD molecules were detected by flow cytometry;B:The differences of CD molecules on different cells were analyzed by histogram.Compared with the THP-1-Mφ,* P 0.05,** P 0.01.Fig.3 Differences in the expression of CD16,CD80 ...
After PMA (0, 1, 5, 10, 50, 100 ng/ml) inducement for 48 h,suspended THP-1 cells transferred as adherent macrophages successfully. Confocal laser scanning microscopyshowed that MD -2 mainly expressed on the cellular membrane and cytoplasm, while increasing inductionconcentrations had no ...
PMA诱导THP-1源巨噬细胞的MMP-1表达