THP-1细胞是从急性单核细胞白血病患者的外周血中分离得到的单核细胞系，为悬浮细胞，它在调控组织稳态、...
THP-1是人单核细胞，经过PMA刺激分化为巨噬细胞。实验中常以0.1ug/ml 的PMA处理THP-1 72h 使其成为巨噬细胞。
Worley JR, Baugh MD, Hughes DA, Edwards DR, Hogan A, Sampson MJ, Gavrilovic J. Metalloproteinase expression in PMA- stimulated THP-1 cells: effects of peroxisome proliferator-activated receptor-gamma (PPAR gamma) agonists and 9-cis-retinoic acid. J Biol Chem 2003;278:51340-6....
方法 首先用 PMA梯度剂量(0,25,50,100,200,400ng/ml)作用于 THP 1细胞 24h,通过 CCK 8法检测细胞活力,再分别采用 0,10,50ng/mlPMA体外刺激 THP 1细胞 24h、48h,流式细胞术检测细胞表面标志物 CD11b、CD14。 结果 梯度 PMA剂量(0-100ng/ml)对细胞活力没有影响(P>005)。10ng/ml、50ng/mlPMA处理...
PMA诱导人单核细胞白血病细胞株THP-1细胞表面标志物CD14和CD11b的表达
Confocal image showing cytoplasmic staining in THP-1 treated cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of ...
in macrophages, such as apolipoprotein-E, matrix metalloproteinase 9 and alpha2 macroglobulin are also induced in the PMA-treated THP-1 cells (THP1PMA ... T Kohro,T Tanaka,T Murakami,... - 《Journal of Atherosclerosis & Thrombosis》 被引量: 324发表: 2004年 Priming of human neutrophil func...
weizhonghua99@126.com. Ox-LDL对PMA诱导的THP-1巨噬细胞自噬的影响 白洁 1 ,孟军 1 ,刘艳辉 2 ,李小红 2 ,危当恒 2 (1.南华大学附属第一医院功能科;2.南华大学心血管疾病研究所, 动脉硬化学湖南省重点实验室,湖南衡阳421001) 摘要:目的探讨氧化型低密度脂蛋白(Ox-LDL)对PMA诱导的THP-1巨噬细胞自噬...
P20. Evaluation of phagocytosis of titanium minute particles by PMA-treated THP-1 cells (macrophage)(Poster Presentation,The 5^ Annual Meeting of Japanese ... Non-small-cell lung cancer (nsclc) tumours with activating mutations of the epidermal growth factor receptor (efgr) tyrosine kinase are ...
Phorbol 12-myristate 13-acetate (PMA) 属于佛波酯类天然产物,是一种 PKC、SphK、NF-κB 的激活剂。Phorbol 12-myristate 13-acetate 可诱导 THP1 细胞分化。 规格价格库存数量 1 mg¥ 436现货 5 mg¥ 1,050现货 10 mg¥ 1,770现货 25 mg¥ 3,730现货 ...