m6A甲基化-常量mRNA +lncRNA甲基化测序（lnc-MeRIP-seq）m6A甲基化-微量mRNA +lncRNA甲基化测序（Micro...
Review: Plant Genome Editing Targeted by RNA-Guided DNA Endonuclease CRISPR/Cas9 CRISPR/Cas9(ClusteredRegularlyInterspacedShortPalindromicRepeat/CRISPRAssociatedProtein9)This review chronicles the development of the research on CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR associated...
研究人员首先利用RNA-Seq发现,衣藻BLZ8的转录水平在强光以及碱性等氧化胁迫条件下都明显升高。接着,研究人员对比了在碱性条件下,过表达藻株BLZ8-OX、缺失突变体blz8以及互补突变体blz8-1(BLZ8)生长变化。BLZ8-OX藻株的生长明显快于对照母株(CC-4533),blz8突变体的生长对氧化胁迫更加敏感,blz8-1(BLZ8) 互补...
S4). GhSMAX1-1 was highly expressed in leaves and its expression level in 20 DPA fibers showed a rapid increase, corresponding to RNA-seq data. GhSMXL3-1 had relatively high expression levels in stems, but its expression level in roots was the highest in the RNA-seq data, with ...
RNA-Seq analysis of bitter gourd fruit transcriptome identifies mature seed-specific expression of Momordica charantia unknown protein 1 (McUNP1) gene and its association with intron retention Kumar Ravichandiran Madasamy Parani Original Article 16 December 2024 A new quantitative method to validate...
通过5'RACE进一步确定了miR408对UCL30、UCL17和UCL8的剪切位点。过表达MIR408可以促进愈伤组织的诱导,而敲除MIR408则导致愈伤组织形成出现部分缺陷。通过RNA-seq分析发现,MIR408主要通过影响植物激素信号转导和苯丙烷类黄酮生物合成途径来调节愈伤组织的形成。该研究为水稻成熟胚愈伤组织的诱导机制提供了新的见解。
4 RNA-seq transcriptome analysis of WT and nac2 plants. a, Comparison of WT and nac2 plants (without any treatment) RNA-seq sequences on the reference genome. The Phred quality score Q20 (99% base call accuracy) and Q30 (99.9% base call accuracy) were used to measure the quality of...
Availability of data and materials The RNA-seq data have been deposited in the Genome Sequence Archive in the National Genomics Data Center under the accession number CRA005390, which is publicly accessible at https://ngdc.cncb.ac.cn/gsa....
NAD tagSeq reveals that NAD+-capped RNAs are mostly produced from a large number of protein-coding genes in Arabidopsis. Proc. Natl Acad. Sci. USA 116, 12072–12077 (2019). Article CAS PubMed PubMed Central Google Scholar Yu, X. et al. Messenger RNA 5′ NAD+ capping is a dynamic...
RNA-seq reads, either generated in this study or obtained from public repository (Supplementary Data1), were first subjected to quality control using FastQC v0.11.8 (https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) and trimming using Trimmomatic v0.39 (HEADCROP:10 LEADING:3 TRAILING...