Using dynamics-based comparisons to predict nucleic acid binding sites in proteins: an application to OB-fold domains . Bioinformatics 2009 ; 25 : 1876 - 1883 . /Zen,A. et al. (2009) Using dynamics-based comparisons to predict nucleic acid binding sites in proteins: an application to OB-...
S. Nucleic acid recognition by OB-Fold proteins. Annu. Rev. Biophys. Biomol. Struct. 32, 115–133 (2003). Article CAS PubMed PubMed Central Google Scholar Nomanbhoy, T. et al. Simultaneous binding of two proteins to opposite sides of a single transfer RNA. Nat. Struct. Biol. 8, ...
However, it is preferred to produce the variants that can be used in the context of the invention by genetic engineering, in particular by integrating a nucleic acid sequence encoding said polypeptide into an expression vector. This expression vector is then introduced into a host cell (bacteria ...
We have used the OB-fold, a motif known to promote protein鈥搉ucleic acid interactions, to validate our approach. Results: We have tested the method using this well-characterized nucleic acid binding family. Protein regions consensually involved in statistically significant dynamics-based alignments ...
S1 domainnucleic acid‐binding proteinstaxonomic distributionstructural repeatsProteins of the nucleic acid-binding proteins superfamily perform such functions as processing, transport, storage, stretching, translation, and degradation of RNA. It is one of the 16 superfamilies containing the OB-fold in ...
Structural and biochemical studies on the human Stn1 and Ten1 complex revealed its mechanism of assembly and nucleic acid binding. However, little is known about the structural organization of the multidomain Ctc1 protein and how each of these domains contribute to telomere length regulation. Here...
Consistent with a function in protein-protein interaction, the residues common to Est3 and Tpp1 map to one face of an OB-fold model structure, away from the canonical nucleic acid binding surface. We propose that Est3 and the OB-fold domain of Tpp1 mediate a conserved function in ...
Nucleic acid recognition by OB-fold proteins. Annu. Rev. Biophys. Biomol. Struct. 2003, 32, 115-133. Int. J. Mol. Sci. 2009, 10 2428 16. Alexandrescu, A.T.; Jaravine, V.A.; Dames, S.A.; Lamour, F.P. NMR hydrogen exchange of the OB-fold protein LysN as a function of de...
Structural Classification of Proteins (SCOP) database broadly classifies OB fold proteins into 18 different superfamilies, including nucleic acid-binding superfamily (NAB). The NAB is further divided into 17 families together with cold shock DNA-binding protein family (CSDB). The CSDB have more than...