(1993) Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein. Nature, 364, 164-168.Schindelin, H., Mahariel, M.A. & Heinemann, U. (1993) Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major ...
We also analysed the nucleic acid and RNAP II binding properties of the KOW domains. KOW4 variants interacted with nucleic acids, preferentially single stranded RNA, whereas no nucleic acid binding could be detected for KOW6-7. Weak binding of KOW4 to the RNAP II stalk, which is comprised...
Second, the target nucleic acid must be prepared as a single-stranded nucleic acid, and this preparation can be laborious and time consuming. In recent years, for genome editing, many programmable nucleic acid-binding proteins, such as the zinc-finger protein (ZFP), transcription activator-like ...
A systematic search for sequences that potentially could form metal-binding domains in proteins has been performed. Five classes of proteins involved in nucleic acid binding or gene regulation were found to contain such sequences. These observations suggest numerous experiments aimed at determining wheth...
Prediction of DNA interaction sites in nucleic-acid-binding proteins based on phosphate-binding helix-turn-helix module. Prediction of DNA interaction sites in nucleic-acid-binding proteins based on phosphate-binding helix-turn-helix module. Yura K. , Go M. Biophysics 38(Supple 2), S166, 1998...
Nucleic acid binding proteins are mostly composed of at least one DNA/RNA-binding domain where the interfacing with amino acids take place in specific or nonspecific manner. From: Encyclopedia of Bioinformatics and Computational Biology, 2019
The Alexa Fluor, Pacific Blue and Pacific Orange Antibody Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provide researchers ...
Nucleic acid binding causes conformational changes because the trigger domain acts as a ‘roadblock’ that prevents the guide RNA 5′ ends and the target DNA 3′ ends from reaching their canonical pockets; this disorders the MID domain and promotes Crt-SPARTA dimerization. Two RNA–DNA-loaded ...
We term this domain the Central Non-core Domain (CND; residues 87-217). We show how the CND alone, and in combination with other regions of non-core RAG1, functions in nuclear localization, zinc coordination, and interactions with nucleic acid. Together, these results demonstrate the multiple...
1. A nucleic acid binding polypeptide that comprises a succession of at least 12 modules, wherein each module consists of 30 to 35 contiguous amino acid residues, wherein said succession of modules displays a base per base specificity towards a nucleic acid target sequence, wherein at least on...