Provided herein are polypeptides, compositions comprising the polypeptides and methods for genome editing and gene regulation (e.g., activation and/or repression) using the polypeptides or the compositions comprising the polypeptides, such as, DNA binding domains derived from the genus of Ralstonia. ...
We also analysed the nucleic acid and RNAP II binding properties of the KOW domains. KOW4 variants interacted with nucleic acids, preferentially single stranded RNA, whereas no nucleic acid binding could be detected for KOW6-7. Weak binding of KOW4 to the RNAP II stalk, which is comprised...
Nucleic acid binding by LIM domains, while suggested by structural considerations, remains an unproven possibility. 核酸结合的林域,而所建议的结构性因素,仍然是一个未经证实的可能性。 www.syyxw.com 2. Nucleic Acid Binding Protein of Bombyx mori Cytoplasmic Polyhedrosis Virus 家蚕细胞质型多角体病毒的...
The invention provides a method for preparing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding...
et al. In silico evolution of nucleic acid-binding proteins from a nonfunctional scaffold. Nat Chem Biol 18, 403–411 (2022). https://doi.org/10.1038/s41589-022-00967-y Download citation Received30 March 2021 Accepted04 January 2022 Published24 February 2022 Issue DateApril 2022 DOIhttps:/...
The single-stranded nucleic acid-binding protein KHSRP (KH-type splicing regulatory protein) modulates RNA life and gene expression at various levels. KHSRP controls important cellular functions as different as proliferation, differentiation, metabolism, and response to infectious agents. We summarize and...
Nucleic acid binding protein 1 (or oligonucleotide/oligosaccharide-binding fold-containing protein 2A; NABP1) gene is located at chromosome 2q32.3 and comprises seven exons spanning 10.4 kb [115]. OB-binding fold domains are present in multiple proteins that play an essential role in DNA damage ...
The three-dimensional structure of CspB, a prototype of this protein family, revealed that this hydrophilic CSD domain creates a surface rich in aromatic and basic amino acids that may act as the nucleic acid-binding site. We have analysed the potential role of conserved aromatic and basic ...
IFI16 (p204 in mice), a member of the ALR family, contains a pyrin structural domain (PYD) and two DNA-binding hematopoietic interferon-inducible nuclear antigens with 200-amino-acid repeat (HIN) structural domains. IFI16 also binds to dsDNA in a length-dependent manner92. When binding dsDN...
The trigger domain seals the exit of the target DNA–RNA-binding tunnel. Extended Data Fig. 5 Preparation of the Crt-SPARTA heterodimer and the quaternary protein–nucleic acid complex containing the target DNA and the guide RNA. a, Schematic of Crt-SPARTA system protein domains, and the ...