Provided herein are polypeptides, compositions comprising the polypeptides and methods for genome editing and gene regulation (e.g., activation and/or repression) using the polypeptides or the compositions comprising the polypeptides, such as, DNA binding domains derived from the genus of Ralstonia. ...
NMR structure, because it has a unique three-dimensional structure composed of 5 helices (II of Fig. 1C), as well as large negatively-charged surface, which is fundamentally different from the classic nucleic acid binding domains such as RRM (I and III of Fig. 1C) and zinc finger folds...
We also analysed the nucleic acid and RNAP II binding properties of the KOW domains. KOW4 variants interacted with nucleic acids, preferentially single stranded RNA, whereas no nucleic acid binding could be detected for KOW6-7. Weak binding of KOW4 to the RNAP II stalk, which is comprised...
Binding domains capable of detectably binding to a nucleic acid target by watson-crick base pairing and by hoogsteen base pairing 来自 Google Patents 喜欢 0 阅读量: 29 公开/公告日期: Oct 7, 1997 发明人: ET Kool 被引量: 127 摘要: The present invention provides stem-loop and circular ...
The invention provides a method for preparing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding...
In nature, genomes have been optimized by the evolution of their nucleic acid sequences. The design of peptide-like carriers as synthetic sequences provides a strategy for optimizing multifunctional targeted nucleic acid delivery in an iterative process.
Nucleic acid binding by LIM domains, while suggested by structural considerations, remains an unproven possibility. 核酸结合的林域,而所建议的结构性因素,仍然是一个未经证实的可能性。 www.syyxw.com 2. Nucleic Acid Binding Protein of Bombyx mori Cytoplasmic Polyhedrosis Virus 家蚕细胞质型多角体病毒的...
et al. In silico evolution of nucleic acid-binding proteins from a nonfunctional scaffold. Nat Chem Biol 18, 403–411 (2022). https://doi.org/10.1038/s41589-022-00967-y Download citation Received30 March 2021 Accepted04 January 2022 Published24 February 2022 Issue DateApril 2022 DOIhttps:/...
Nucleic acid binding protein 1 (or oligonucleotide/oligosaccharide-binding fold-containing protein 2A; NABP1) gene is located at chromosome 2q32.3 and comprises seven exons spanning 10.4 kb [115]. OB-binding fold domains are present in multiple proteins that play an essential role in DNA damage ...
Using a dot-blot of a selected set of lipids one can determine the lipid-binding specificity of a particular target protein. In addition, it can be used to identify the lipid-binding domains within a target protein. Moreover, the assay permits an estimation of the affinity constant. ...