Polypeptides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided. Methods of promoting the annealing of complementary nucleic acid strands are provided. Methods of increasing the processivity of a DNA polymerase are provided. Methods of enhancing ...
A method of stabilizing an DNA:RNA duplex comprising combining the DNA:RNA duplex with a polypeptide comprising an amino acid sequence of a nucleic acid binding polypeptide or a fragment thereof having nucleic acid binding activity, wherein the polypeptide comprises an amino acid sequence having at...
Variant Gal6 proteins that fail to bind nucleic acid also exhibit reduced ability to protect cells from bleomycin toxicity, suggesting that the nucleic acid binding activity of Gal6p is important in bleomycin detoxification and may be involved in its normal biological functions. 展开 ...
Nucleic acid binding causes conformational changes because the trigger domain acts as a ‘roadblock’ that prevents the guide RNA 5′ ends and the target DNA 3′ ends from reaching their canonical pockets; this disorders the MID domain and promotes Crt-SPARTA dimerization. Two RNA–DNA-loaded ...
However, inclusion of the IDRs to any of the structural domains resulted in significantly increased binding affinity and binding cooperativity towards a poly (U) ssRNA under in vitro conditions (KD ∼ 0.8 μM), suggesting that the IDRs are able to modulate the nucleic acid binding activity of...
Single substitutions of three highly conserved phenylalanine residues (Phe-15, Phe-17, Phe-27) by alanine and substitution of one histidine (His-29) by glutamine, all located within the putative RNA-binding sites RNP-1 and RNP-2, abolished the nucleic acid-binding activity of CspB. ...
Fig. 3 N-terminal 152 amino-acid residue-long domain of DHX30 (DHX30N), which has dsRNA-binding activity, is necessary and sufficient for binding the NS1 protein and inhibiting virus replication. 为了鉴定最可能与dsRNA相互作用的氨基酸残基,...
b, Comparison of nucleic acid binding SEPROs and PPRs (designated by their UniProt IDs). c, The variant masking scheme used in the experiment shown in Fig. 6b. Extended Data Fig. 2 Electrophoretic mobility shift assay of SEPRO5. Incubation of SEPRO5 with a selection of RNA and DNA ...
So can ATP also bind nucleic-acid-binding proteins without RRM fold? Here we characterized the interaction between ATP and SYNCRIP acidic domain (AcD), a non-canonical RNA-binding domain with no similarity to RRM fold in sequence and structure. The results reveal that ATP does bind AcD at ...
nucleic acid 基本解释 名词核酸 nucleic acid 网络解释 1. nucleic acid在线翻译 1. 核酸 核酸:生物技术和生物工程(Bioengineering)这(2)核酸 核酸(nucleic acid)最初从细胞核中分离出来,故得此名. 酸可分为两类,即脱氧核糖核酸(DNA)和核糖核酸(RNA). 它们都是高聚1953 年沃森(Watson)和克里克(Crick)提出DNA...