Here, we propose to use this method to predict protein regions involved in the binding of nucleic acids. We have used the OB-fold, a motif known to promote protein鈥搉ucleic acid interactions, to validate our approach. Results: We have tested the method using this well-characterized nucleic...
Nucleic acid binding protein 1 (or oligonucleotide/oligosaccharide-binding fold-containing protein 2A; NABP1) gene is located at chromosome 2q32.3 and comprises seven exons spanning 10.4 kb [115]. OB-binding fold domains are present in multiple proteins that play an essential role in DNA damage ...
The protein component PhoRpp38 of Pyrococcus horikoshii ribonuclease P (RNase P) is known to be a multifunctional RNA-binding protein. Previous biochemical... JD Puglisi,JR Williamson - 《Current Opinion in Structural Biology》 被引量: 0发表: 2012年 Nucleic acid recognition by OB-fold proteins....
Sequence and structure-based alignments revealed a possible OB-fold single-strand nucleic acid-binding motif in Rpb7. Purified Rpb4-Rpb7 complex exhibited both single-strand DNA- and RNA-binding activities, and a small deletion in the putative OB-fold nucleic acid-binding surface of Rpb7 ...
Nucleic acid sensing in HSPC gene therapy DNA damage responses Restriction factors How to overcome cell intrinsic hurdles to gene engineering in HSPC Conclusions and perspectives References Acknowledgements Author information Ethics declarations Additional information Rights and permissions About this article This...
[46]. Functionalization the surfaces of SNA with aptamer sequences as a nucleic acid shell is another improving mechanism for targeting and biodistribution of SNAs. In a study, Caroline D. et al. reported that functionalization protein(β-galactosidase)-SNA with DNA aptamers binding to transferrin...
However, the combination of free chemotherapeutic drug and gene loaded nanocarrier has some weaknesses: 1) it is difficult to deliver controllable ratios of drug to nucleic acid in the same target cells; 2) difficulty in synchronizing pharmacokinetics and biodistribution; and 3) unwanted toxicity in...
Nucleic acid therapeutics open almost infinite possibilities. They can be designed to i) restore the normal biological functions of deficient proteins; ii) halt the production of abnormal deleterious protein while leaving all other proteins unaffected; iii) increase the synthesis of a protein of intere...
The lab advanced the pDNA binding carrier structures by designing amidated PEG-poly(aspartic acid) (pAsp) derivatives with good buffering capacity in the endolysosomal range, yielding PEG-pAsp(DET) and PEG-pAsp(TEP) as most effective carriers [93]. For further optimization of in vivo delivery,...
1. An isolated nucleic acid molecule that encodes a human 15S-lipoxygenase (15-Lox-2)polypeptide. 2. The nucleic acid molecule of claim 1, wherein the encoded human 15-Lox-2 comprises the amino acid sequence of SEQ ID NO:2. 3. The nucleic acid molecule of claim 2, further defined...