Here, we propose to use this method to predict protein regions involved in the binding of nucleic acids. We have used the OB-fold, a motif known to promote protein鈥搉ucleic acid interactions, to validate our approach. Results: We have tested the method using this well-characterized nucleic...
1). These observations suggest the presence of two different mechanisms of nucleic acid recognition depending on the vector genome structure and replication. RNA sensors Innate immune receptors detecting RNA are referred to as RIG-I like receptors (RLR). The three central members of the mammalian ...
First studies explored the requirements for nucleic acid binding by introducing the cationic amino acids lysine, arginines or ornithines [138,139], disulfide-forming nanoparticle-stabilizing cysteines [140,141], endosomal-buffering histidines [[141], [142], [143], [144], [145], [146], [147...
Nucleic acid therapeutics open almost infinite possibilities. They can be designed to i) restore the normal biological functions of deficient proteins; ii) halt the production of abnormal deleterious protein while leaving all other proteins unaffected; iii) increase the synthesis of a protein of intere...
Generally, the formation of these nanomedicines was firstly constructed the corporate drug pairs (CDP) (Figure 5A), which required certain interactions between small-molecular drug and nucleic acid, like as electrostatic adsorption, coordination bond or covalent coupling; then a biocompatible skeleton ...
[46]. Functionalization the surfaces of SNA with aptamer sequences as a nucleic acid shell is another improving mechanism for targeting and biodistribution of SNAs. In a study, Caroline D. et al. reported that functionalization protein(β-galactosidase)-SNA with DNA aptamers binding to transferrin...
1. A method for enzymatically modifying a conjugate comprising the steps of: providing a conjugate comprising a nucleic acid and a synthetic binding unit, wherein the synthetic binding unit is selected from the group consisting of pRNA, pDNA, and CNA; and contacting the conjugate with at least...
By “complementarity” is meant that a nucleic acid can form hydrogen bond(s) with another nucleic acid sequence by either traditional Watson-Crick or other non-traditional types. In reference to the nucleic molecules of the present invention, the binding free energy for a nucleic acid molecule ...
11. The nucleic acid expression cassette according to claim 10, wherein the therapeutic protein is a clotting factor. 12. The nucleic acid expression cassette according to claim 11, wherein the therapeutic protein is factor IX. 13. A vector comprising the nucleic acid regulatory element of claim...
A key aspect of this invention pertains to the discovery of a novel 15S-lipoxygenase (15-Lox-2) protein and nucleic acid encoding the 15-Lox-2 protein. Preferred nucleic acid and amino acid sequences for 15-Lox-2 are described in SEQ ID NO:1 and SEQ ID NO:2. It is another aspect...