研究发现一些ciRNAs定位在细胞核中,可以与PolⅡ结合通过cis 作用模式调控宿主的转录活性。研究发现一类叫做EIciRNA的环状RNA与RNA PolⅡ关系密切。比如circEIF3J 和circPAIP2,定位在细胞核,与U1小核核糖核蛋白(snRNPs)结合,通过cis-acting模式增强亲本基因的转录。 另外, 有一小部分c
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a single-stranded positive ribonucleic acid (RNA) virus, is the causative agent, and multiple variants have emerged, ranging from alpha to omicron.3SARS-CoV-2, belonging to the Sarbecovirus subgenus of betacoronavirus, possesses a gen...
Two types of small RNA molecules function in RNAi. The first are synthetic, short interfering RNA (siRNA) molecules that target mRNA cleavage, effectively knocking down the expression of a gene of interest. MicroRNA (miRNA) molecules, on the other hand, are naturally occur...
RNA immunoprecipitation (CLIP) assay, which induces irreversible covalent bonds between directly interacting proteins and RNA, as previously reported24. DHX9 or NPM1 antibody-bound complexes exhibited a significant enrichment inKIMAT1(Fig.4c, d).Lnc-CCDSTandSAMD12-As1, two lncRNAs previously reported...
LncRNA Types Long non-coding RNAs (lncRNAs) are classified into five types based on origin, orientation, and position: Intergenic, Intronic, Sense, Antisense, and Bidirectional. They have distinct functions depending on their source. For instance, Antisense lncRNAs can influence alternative splicing ...
The pre-miRNA is further processed in the cytoplasm through the activity of transactivation response element RNA-binding protein (TRBP) and Dicer. A duplex composed of two miRNAs (miRNA/miRNA∗) is created after pre-miRNA loop is cleaved. The strand attaches to Argonaute 2 (AGO2), which ...
The following steps should be performed during SEC of lncRNA: 1. Collect fractions of 0.5ml volume. The homogeneity of the preparation will usually be apparent from the elution chromatogram (Fig. 1B). If the RNA of interest is homogenous and elutes as a single peak, this fraction should ...
《非编码RNA研究(英文)》(Non-coding RNA Research, 简称NCRNA)(季刊),创刊于2016年12月,是由中国科学院主管,科学出版社主办,科爱公司运营的高质量国际开放获取期刊,旨在发表高水平的研究论文和综述,传播前沿研究,展示非编码RNA在现代医学和研究中不断演变的重要性。NCRNA作为一本专注于非编码RNA的英文学术期刊,旨...
piRNAs are the largest class of the small non-coding RNA family and are implicated in epigenetic and post-transcriptional regulation but still lack functional characterization8. lncRNAs are a diverse class of RNAs, believed to have an important role in cellular mechanisms; however, little biological...
The invention is related to LncHIFCAR (long noncoding HIF-1α co-activating RNA)/MIR31HG and its applications in cancer diagnosis, cancer therapy, prognosis predication of a cancer and determination of therapeutic regimen of a cancer. The present invention identifies a hypoxia-inducible lncRNA, ...