研究发现一些ciRNAs定位在细胞核中,可以与PolⅡ结合通过cis 作用模式调控宿主的转录活性。研究发现一类叫做EIciRNA的环状RNA与RNA PolⅡ关系密切。比如circEIF3J 和circPAIP2,定位在细胞核,与U1小核核糖核蛋白(snRNPs)结合,通过cis-acting模式增强亲本基因的转录。 另外, 有一小部分circRNAs是可以翻译的。研究...
Two types of small RNA molecules function in RNAi. The first are synthetic, short interfering RNA (siRNA) molecules that target mRNA cleavage, effectively knocking down the expression of a gene of interest. MicroRNA (miRNA) molecules, on the other hand, are naturally occur...
LncRNA (Long non-coding RNA) is a class of non-coding RNA molecules with lengths exceeding 200 nucleotides and lacking protein-coding functions.
《非编码RNA研究(英文)》(Non-coding RNA Research, 简称NCRNA)(季刊),创刊于2016年12月,是由中国科学院主管,科学出版社主办,科爱公司运营的高质量国际开放获取期刊,旨在发表高水平的研究论文和综述,传播前沿研究,展示非编码RNA在现代医学和研究中不断演变的重要性。NCRNA作为一本专注于非编码RNA的英文学术期刊,旨...
(Fig.1b). To identify potential KRAS-modulated pathways we searched for KRAS-responsive lncRNAs. We carried out RNA sequencing (RNA-seq) analysis after overexpression (OE) of either KRASWTor KRASG12Din H1299 cells, which although harboring an NRAS mutation do not depend on NRAS signaling13....
In addition, lncRNA-KRTAP5-AS1 and lncRNA-TUBB2A could act as competing endogenous RNAs to affect the function of Claudin-4. Our results suggest that non-coding RNAs play important roles in the regulatory network of Claudin-4. As such, non-coding RNAs should be considered as potential ...
为进一步验证LINC01503与SFPQ之间的相互作用,研究者用抗SFPQ抗体进行了RIP实验,发现LINC01503 RNA明显富集(图3D),敲除LINC01503后LINC01503与SFPQ之间的相互作用消失(图3E)。接下来,为确定LINC01503与SFPQ结合所需的特异性片段,研究者构建了一系列LINC01503截短质粒并进行了RNA pull down实验,发现LINC01503的敲除或过...
RNAi sequences were cloned into the GV493 plasmid and expanded inEscherichia coliDH5α cells in house (Fig.S1). The LncZFAS1 RNA sequence was cloned into pcDNA3.1 (Fig.S2). Lentiviral packaging was performed in 293 T cells seeded at 5 × 106cells/15 mL in T75 flasks in DMEM with 10...
When further strokes of pipetting were applied, however, the spectrum rarely changed, if any (Fig. 4a). The decrease in the fluorescence intensity caused by pipetting for all wavelengths, which was observed in the absence of RNA (Fig. 1a), was not seen. This observation indicates that p...
ncRNAs in tumors can target components of the TGF-β signaling pathway or directly regulate TGF-β target gene transcription in different tumors, thus inhibiting the antitumor immune response and promoting TIE (Table6, Fig.5). For example, miR-133,198miR-141,199lncRNA ANRIL,200and lncRNA 0097...