此篇文章摘录于Methods in Molecular Biology 2032中的一篇,名为Multiparametric Flow Cytometry Analysis of Naive, Memory,and Effector T Cells(https://doi.org/10.1007/978-1-4939-9650-6_8 )其作者是Ankit Saxena等。 自然杀伤细胞(NK)是与T细胞和B细胞同一家族的淋巴细胞,来自共同的祖细胞。它可以杀死病毒感...
NK细胞源自CD34+造血祖细胞(Hematopoietic Progenitor cells, HPCs),发育为淋巴样祖细胞(Common Lymphoid Progenitor, CLP)后,逐渐下调CD34、上调CD56,进而发育为NK细胞。NK细胞主要存在于淋巴结、骨髓、外周血、肺、脾脏、肝脏等器官内;根据CD56在细胞表面表达密度的多少,NK细胞包...
此篇文章摘录于Methods in Molecular Biology 2032中的一篇,名为Multiparametric Flow Cytometry Analysis of Naive, Memory,and Effector T Cells(doi.org/10.1007/978-1-4)其作者是Ankit Saxena等。 自然杀伤细胞(NK)是与T细胞和B细胞同一家族的淋巴细胞,来自共同的祖细胞。它可以杀死病毒感染的和恶变的细胞,这个...
NK细胞源自CD34+造血祖细胞(Hematopoietic Progenitor cells, HPCs),发育为淋巴样祖细胞(Common Lymphoid Progenitor, CLP)后,逐渐下调CD34、上调CD56,进而发育为NK细胞(图2)。NK细胞主要存在于淋巴结、骨髓、外周血、肺、脾脏、肝脏等器官内;根据CD56在细胞表面表达密度的多少,NK细胞包括CD56bright和CD56dim两个亚群,其...
Flower cells, Sezary cells, and prolymphocytes are useful morphological clues for diagnosis. Immunophenotyping by flow cytometry, along with clinicopathologic correlation, is crucial for the diagnosis and subclassification of MTNKN. All subtypes except T-LGLL show inferior...
Flow Cytometry View Larger Intracellular NK Cell analysis over a 14-day time course.NK cells were expanded from PBMCs using plate-bound Anti-Human NKp46 Monoclonal Antibody (MAB1850) in ExCellerate™ Human NK Cell Expansion Media, Xeno-Free (CCM032) plus Recombinant Human IL-2 (27 ng/mL,...
Flow Cytometry-Based assay to analyze Natural Killer Cells capability to target and kill cells of interest Key Benefits • Flow Cytometry-Based NK Killing Assay • LAMP proteins used for a direct method to evaluate cytotoxicity • Rapid, Consistent Analysis of CAR-NK or NK Cells ...
NK cells can be further characterized into a variety of subsets through many different cell surface markers including CD16 and the KIR family of receptors. Check out these tools that can be used for NK cell analysis including flow cytometry, functional assays,...
参考文献：[1] Zotto G D , Antonini F , Pesce S , et al. Comprehensive Phenotyping of Human PB NK Cells by Flow Cytometry[J]. Cytometry Part A, 2020, 97(9).[2] Traba J , Waldmann T A , Anton O M . Analysis of Human Natural Killer Cell Metabolism[J]. Journal of ...
参考文献:Del Zotto G, Antonini F, Pesce S, Moretta F, Moretta L, Marcenaro E. Comprehensive Phenotyping of Human PB NK Cells by Flow Cytometry [published online ahead of print, 2020 Mar 21].Cytometry A. 2020;10.1002/cyto.a.24001. doi:10.1002...