Flow Cytometry View Larger Intracellular NK Cell analysis over a 14-day time course.NK cells were expanded from PBMCs using plate-bound Anti-Human NKp46 Monoclonal Antibody (MAB1850) in ExCellerate™ Human NK Cell Expansion Media, Xeno-Free (CCM032) plus Recombinant Human IL-2 (27 ng/mL,...
设门 参考文献:Creegan M, Degler J, Paquin-Proulx D, Eller MA, Machmach K. OMIP-098: A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping [published online ahead of print, 2023 Oct 8]. Cytometry A. 2023;10.1002/cyto.a.24802. doi:10.1002/cyto.a.24802...
图5.表征人类NK细胞自然杀伤功能的panel 三、细胞共培养杀伤能力鉴定：1、用GFP，CFSE等带荧光的染料去标记NK的靶细胞；2、将NK细胞与靶细胞的共培养（不同的靶细胞，培养比例梯度和培养时间需要预实验摸索）；3、收样后，用CD56流式抗体标记NK细胞，结合绝对计数微球，对靶细胞的数量检测，进行统计分析 图6.细胞...
参考文献: [1] Zotto G D , Antonini F , Pesce S , et al. Comprehensive Phenotyping of Human PB NK Cells by Flow Cytometry[J]. Cytometry Part A, 2020, 97(9). [2] Traba J , Waldmann T A , Anton O M . Analysis of Human Natural Killer Cell Metabolism[J]. Journal of Visualized ...
OMIP‐080: 29‐Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantationThis 29‐color panel was developed and optimized for the monitoring of NK cell and T cell reconstitution in peripheral blood of patients after HSCT. ...
自然杀伤细胞(natural killer cell,NK),是固有免疫细胞中的重要成员,无需抗原预致敏就能破坏靶细胞,且无MHC限制性,应答速度快,在免疫应答的早期即可发挥作用。最近也有研究显示,NK细胞也能展现部分适应性免疫细胞的一些特性。NK细胞表面不表达多态性克隆型受体,如t细胞受体(TCR)或b细胞受体(BCR),它们的细胞杀伤功能...
信源:Hertoghs N, Schwedhelm KV, Stuart KD, McElrath MJ, De Rosa SC. OMIP-064: A 27-Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells [published online ahead of print, 2020 May 16]. Cytometry A...
This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol ...
Here a simple and reliable method is described to analyze different NK cell functions using a flow cytometry-based assay. NK cell functions can be evaluated not only for the whole NK cell population, but also for different NK cell subsets. This technique enables scientists to easily study NK ...
Reference:Development of a 43 color panel for the characterization of conventional and unconventional T-cell subsets,B cells, NK cells, monocytes, dendritic cells, and innate lymphoid cells using spectral flow cytometry 一、背景信息 二、光谱方案 ...