设门 参考文献:Creegan M, Degler J, Paquin-Proulx D, Eller MA, Machmach K. OMIP-098: A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping [published online ahead of print, 2023 Oct 8]. Cytometry A. 2023;10.1002/cyto.a.24802. doi:10.1002/cyto.a.24802...
图5.表征人类NK细胞自然杀伤功能的panel 三、细胞共培养杀伤能力鉴定：1、用GFP，CFSE等带荧光的染料去标记NK的靶细胞；2、将NK细胞与靶细胞的共培养（不同的靶细胞，培养比例梯度和培养时间需要预实验摸索）；3、收样后，用CD56流式抗体标记NK细胞，结合绝对计数微球，对靶细胞的数量检测，进行统计分析 图6.细胞...
自然杀伤细胞(natural killer cell,NK) 是固有免疫细胞中的重要成员,无需抗原预致敏就能破坏靶细胞,且无MHC限制性,应答速度快,在免疫应答的早期即可发挥作用。最近也有研究显示,NK细胞也能展现部分适应性免疫细胞的一些特性。NK细胞表面不表达多态性克隆型受体,如t细胞受体(TCR)或b细胞受体(BCR),它们的细胞杀伤功能...
自然杀伤细胞(natural killer cell,NK),是固有免疫细胞中的重要成员,无需抗原预致敏就能破坏靶细胞,且无MHC限制性,应答速度快,在免疫应答的早期即可发挥作用。最近也有研究显示,NK细胞也能展现部分适应性免疫细胞的一些特性。NK细胞表面不表达多态性克隆型受体,如t细胞受体(TCR)或b细胞受体(BCR),它们的细胞杀伤功能...
参考文献:Pomplun N, Weisgrau KL, Evans DT, Rakasz EG. OMIP-028: Activation Panel for Rhesus Macaque NK Cell Subsets. Cytometry Part A : the journal of the International Society for Analytical Cytology. 2015;87(10):890-893. doi:10.1002/cyto.a.22727. ...
Today, three different types of NK cell memory have been described: (i) cytokine-induced memory-like cells, (ii) MCMV-induced memory, and (iii) liver-restricted NK cell memory (Fig. 5.4). Sign in to download full-size image Figure 5.4. Three forms of NK cell memory. (left panel) NK...
Reference:Development of a 43 color panel for the characterization of conventional and unconventional T-cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate lymphoid cells using spectral flow cytometry 一、背景信息 二、光谱方案 ...
(d) Ex vivo cytotoxicity assay: NK cells were isolated from the peripheral blood from patients before start of the therapy and 24 hours after the end of first AFM13 infusion to assess the cytotoxicity against the Hodgkin lymphoma cell line L428 (left panel). Left panel: one representative ...
2.该抗体适用于多种科研实验,包括BCIP/NBT,AP(Affinity Purification),B/N(Blocking/Neutralize),CD(Cell Depletion),ELISA(Cap),ELISA(Det),ELISA(Comp),FC(Flow Cytometry),WB(Western Bolt)等。 3.公司抗体种类齐全,包括一抗,标记一抗,二抗,标记二抗,酶标记二抗,生物素二抗,荧光色标记二抗,胶体金标二抗,亲...
aBALB/c mice were treated with PBS, YB1, or YB1 plus NK cell depletion antibody, respectively. NK cell depletion efficiency was validated on days 2, 8, and 14 by flow cytometry. 4T1 cancer cells were i.v. implanted on day 4.bPercentage of peripheral blood NK cells at the indicated ...