受此启发,研究者们沿袭相似的工程化改造技术以探索其他免疫细胞的临床转化可能性,代表性的包括肿瘤浸润淋巴细胞(Tumor Infiltrating Lymphocyte, TIL)、自然杀伤细胞(Natural Killer Cell, NK Cell)、恒定自然杀伤T细胞(Invariant Natural Killer T Cell, iNKT Cell)、调节性T细胞...
图片来源:Cell, doi: 10.1016/j.cell.2020.02.029; 康橙投资 (二)NK细胞的表型及特征 基于细胞表面蛋白表达对细胞进行免疫分型是生物医学研究中的常用方法,实践中多采用流式细胞技术(Flow Cytometry),分型依据是免疫荧光检测信号的有无(采用“+/-”或“positive/negative”表示)以及相对强度(采用“+/++/+++”或...
Though we recently found some reports on sterile sorting of natural killer cells (NK cells) by flow cytometer (FCM), which manifested cytotoxicity, no reports have concerned antineoplasticity of sterile NK cells on glioma cell lines and cultured surgical materials of brain tumor so far as we ...
人外周血中分离NK细胞、体外扩增及功能检测 自然杀伤细胞（natural killer cell，NK），是固有免疫细胞中的重要成员，无需抗原预致敏就能破坏靶细胞，且无MHC限制性，应答速度快，在免疫应答的早期即可发挥作用。最近也有研究显示，NK细胞也能展现部分适应性免疫细胞的一些特性。NK细胞表面不表达多态性克隆型受体，如t细...
3. Atkuri KR, Stevens JC, Neubert H. Mass cytometry: a highly multiplexed single-cell technology for advancing drug development. Drug Metab Dispos. 2015 Feb;43(2):227-33. doi: 10.1124/dmd.114.060798. 4. Kay AW, Strauss-Albee DM, Blish CA. Application of Mass Cytometry (CyTOF) for Func...
FlowX Human NK Cell Killing Flow Cytometry Kit Summary Flow Cytometry-Based assay to analyze Natural Killer Cells capability to target and kill cells of interest Key Benefits • Flow Cytometry-Based NK Killing Assay • LAMP proteins used for a direct method to evaluate cytotoxicity ...
参考文献:Creegan M, Degler J, Paquin-Proulx D, Eller MA, Machmach K. OMIP-098: A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping [published online ahead of print, 2023 Oct 8]. Cytometry A. 2023;10.1002/cyto.a.24802. doi:10.1002/cyto.a.24802 ...
This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol ...
A Quantitative Flow Cytometry-Based Method for Autophagy Detection Across the Cell Cycle Autophagy is deregulated in cancer cells and often activated as a cellular stress response to anticancer therapies. Flow cytometry-based assays enable dete... VO Kaminskyy - 《Methods in Molecular Biology》 被引...
3. Atkuri KR, Stevens JC, Neubert H. Mass cytometry: a highly multiplexed single-cell technology for advancing drug development. Drug Metab Dispos. 2015 Feb;43(2):227-33. doi:10.1124/dmd.114.060798 4. Kay AW, Strauss-Albee DM, Blish CA. Application of Mass Cytometry (CyTOF) for Function...