The major immune cell lineages were detected by flow cytometry. t-Distributed stochastic neighbor embedding (t-SNE) and statistical analysis were used to analyze the composition of immune cells in tumor microenvironment of CRC. To analyze the phenotype of NK cells,...
建立改良型人NK细胞体外扩增技术_李亚芬
Through this effort we provide ranges of NK cell surface receptor expression for a local adult population as well as provide insight into intra-individual variation. 展开 关键词: NK cells, immunological phenotype, primary immune deficiency, flow cytometry ...
Dead K562 cell populations were detected by FACS calibour flow cytometr, and the data obtained was analyses on cell-Quest software. The proportion of CD16/CD56cells was then calculated. Results: The proportion of NK cells were 9.21% ± 3.06 and 13.48% ± 4.09 in RSA and healthy women, ...
After 5 h, effector cell–induced cell death (Topro3+) of target cells was determined by flow cytometry (n = 5). (f) Splenic and VAT-resident NK cells were stimulated in vitro with IL-15 or agonistic NCR1 antibodies, and expression of Ki67 was analyzed after 48 h. Gated for CD3–...
NK cells were enriched and analysed for their specific immunophenotype by flow cytometry. This analysis demonstrated the successful enrichment of cell populations with phenotype CD3−/CD16+/CD56+ by all blood samples (Fig. 1A). The gating strategy was reported into the Additional file 1 for comp...
DX5+NKT cells are characterized by expression of the NK cell marker DX5 in the context of CD3. However, little is known about the phenotype and functional capacity of this unique cell population. Therefore, we investigated the expression of several T cell and NK cell markers, as well as ...
I–K Flow cytometry phenotype of engineered iNK cells following 39 days of NK cell differentiation (n = 5 independent experiments; multiple unpaired two-tailed t-tests) as well as following 2 additional weeks of iNK cell expansion (n = 4 independent experiments; multiple unpaired two...
We showed that mNKp46 W32R can be expressed on the cell surface, however unstably, that it functions similarly to WT mNKp46, that it is arrested in the ER, and is aberrantly glycosylated27. These findings indicate that the Noé phenotype probably stems from the aberrant expression of mNK...
Hijacking the TGFβRII receptor promotes NK cell activation. Upon the recognition of TGFβ by the wild-type TGFβRII receptor, a signaling cascade is initiated, resulting in a compromised NK cell phenotype and reduced cytotoxicity. A dominant-negative mutant in which most of the intracellular doma...