Cellular uptake - Confocal microscopyFlow cytometryFor several years, two advanced techniques, confocal laser scanning microscopy (CLSM) and flow cytometry, in particular fluorescence-activated cell sorting (FACS) have been used more and more to study the cellular uptake of liposomal drug delivery ...
A.F.L.S. cloned, expressed, purified and characterized proteins, synthesized and characterized peptides and protein–peptide conjugates, and performed uptake, cell viability, microscopy and flow cytometry experiments. M.L. performed microscopy experiments and wrote the quantification script together with...
cellular uptakedithionitelipid-bindingstructure-activity relationshipThe pAntp peptide, corresponding to the third helix of the Antennapedia homeodomain, is internalized by a receptor-independent process into eucaryotic cells. The precise mechanism of entry remains unclear but the interaction between the ...
Urinary crystals with various sizes are present in healthy individuals and patients with kidney stone; however, the cellular uptake mechanism of calcium oxalate of various sizes has not been elucidated. This study aims to compare the internalization of nano-/micron-sized (50 nm, 100 nm, and...
(B) Dextran uptake by HeLa cells in the presence of increasing concentrations of EVs, as quantified by flow cytometry. Values represent mean + S.E.M. *p < 0.05. Due to their reported low specificity in some cell types, chemical inhibitors alone may not be sufficient to fully elucidate the...
flow cytometryIn vitro and in vivo behavior of nanoparticles (NPs) is often studied by tracing the NPs with fluorescent dyes. This requires stable incorporation of dyes within the NPs, as dye leakage may give a wrong interpretation of NP biodistribution, cellular uptake, and intracellular ...
susceptibility was determined by MIC and MBC assays, and time–kill curve experiments, while the cellular uptake was evaluated by monitoring the fluorescence of the complexes remaining in the supernatant of the cultures after incubation for various periods of time, flow cytometry and confocal ...
Cellular barcoding in flow cytometry extends the utility of the technique for high-dimensional multi-pass single-cell analyses. Fluorescence-based flow cytometry has been a workhorse in the single-cell analysis of surface markers, intracellular cytokines, intranuclear proteins (such as transcription ...
Flow cytometry analysis of EV uptake In the in vitro experiments, liver cells were detached from the plate surface with Accutase solution (Sigma, USA) and were analyzed directly after a centrifugation step (50×g, 3 min at 4 °C). NPC cells were separated from HEPs by centrifugation. The ...
Effect of SDC domains on α−syn and tau fibril uptake. (a) Flow cytometry histograms representing HS expression of WT K562 cells or SDC transfectants after NaClO3 treatment. (b,c) Flow cytometry histograms representing intracellular fluorescence of fluorescent (FITC) fibril (α−syn or tau)...