破解谜团,究竟是什么影响了NK细胞的ADCC? 本文故事情节纯属虚构,仅为便于大家理解其中的专业内容,相关知识点和图片引用自Benavente MCR, Hakeem ZA, Davis AR, et al. Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC. Sci Rep. 2024;14(1):7938. Published...
NK cells can be further characterized into a variety of subsets through many different cell surface markers including CD16 and the KIR family of receptors. Check out these tools that can be used for NK cell analysis including flow cytometry, functional assays,...
Fauriat, J. M. Nunes et al., "Functional anal- ysis of human NK cells by flow cytometry," Methods in Molecular Biology, vol. 612, pp. 335-352, 2010.Functional analysis of human NK cells by flow cytometry. Bryceson,Y. T. et al. Methods in Molecular Biology . 2010...
NK cells migrating on developmentally supportive stroma adopted a broad leading edge and a uropod-like trailing end. Surprisingly, however, when CD56brightNK cells were found in arrest, they were conjugated to stromal cells with firm adhesion and a polarized phenotype (Fig. 2a). The addition of ...
Increased Numbers of NK Cells, NKT-Like Cells, and NK Inhibitory Receptors in Peripheral Blood of Patients with Chronic Obstructive Pulmonary Disease The frequencies of CD3+ T, CD4+ T, CD8+ T, B, NK, and NKT-like cells were determined using flow cytometry. The frequencies of spontaneous and...
In wildtype mice, this expansion is rather modest, as 40–50% of all NK cells express the Ly49H receptor (Dokun et al., 2001). Therefore, it is unclear if the same type of memory phenotype would develop without a substantial clonal expansion. While there are a few markers described to...
Representative flow cytometry plots of functional markers expressed on NK cells (lower panel). Healthy controls (Day 0 n = 30, Day 10 n = 30, Day 21 n = 26, Day 35 n = 35), PLWH (Day 0 n = 25, Day 10 n = 24, Day 21 n = 24, Day 35 n...
体外扩增培养的nkinkt细胞及...前言 NK细胞(natural killer),即自然杀伤细胞,是机体固有免疫的组成细胞。NK细胞主要来源于骨髓,在骨髓微环境中逐渐发育成熟,分布至外周血中。当病毒感染细胞、寄生虫侵入等感染因素发生及自身细胞衰老、恶变时,NK细胞可以识别这些异常的细胞,通过穿孔素/颗粒酶途径直接裂解异常细胞...
NK-1.1 and DX5 are commonly used as mouse NK cell markers. Product Details Technical data sheet Product Details Isotype Control PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl Verified Reactivity Mouse Antibody Type Monoclonal Host Species Mouse Immunogen NK-1+ cells from mouse spleen...
We applied the product rule to all co-expression possibilities of two and three KIRs, and performed a linear regression of the observed (from the flow cytometry data) and expected (given by the product rule) frequencies of KIR expression (Figures 3b and c). On uterine NK cells, co-...