Real-time monitoring of inflammation status in 3T3-L1 adipocytes possessing a secretory Gaussia luciferase gene under the control of nuclear factor-kappa B response element. Biochem Biophys Res Commun. 2012 Apr 13;420(3):623-7. doi: 10.1016/j.bbrc.2012.03.049. Epub 2012 Mar 17. ...
The TLR4 reporter cell line is a stably co-transfected cell line which expresses full-length human Toll-like receptor 4 (TLR4) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. The TLR4 reporter cell line has been validated...
Human Embryonic Kidney (HEK/293) cells were stably transfected with a vector carrying EGFP or d2EGFP under control of a synthetic promoter containing 4 copies of the NF-kB response element. Treatment with tumor necrosis factor 伪 (TNF-伪) gave rise to substantial EGFP/d2EGFP expression in up...
driven by four copies of the NF-kB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-kB transcription factors bind to the DNA response elements, inducing transcription of the luciferase ...
NFkBisactivatedandtranslocatesfromcytoplasmtonucleus,whereNFkBbindstoitsresponseelementonthepromoterregionandregulatesawidespectrumofgeneexpression.DysfunctionofNFkBactivityisassociatedwithcancer,inflammatoryandautoimmunedisease,andviralinfection.MonitoringtheNFkBactivityisessentialtounveilthemechanismofthesediseasesandconduct...
element located upstream of a minimal promoter. The NF-kB reporter is premixed with constitutively-expressing Renilla luciferase vector, whichserves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing ...
The pGL4.32[luc2P/NF-κB-RE/Hygro] plasmid containing five copies of an NF-kB response element was purchased from Promega (Promega Corp., Madison, WI, USA). Luciferase activity was measured in samples containing equivalent amounts of protein using a luminometer and luciferase assay reagents. DP...
To measure NF- kB activation, a plasmid containing five copies of an NF-kB response element driving expression of the luciferase reporter gene luc2P was purchased from Promega (Cat# E8491). Relative luminescence was normalized to a co-transfected Renilla luciferase construct, derived from the ...
(interferon-stimulated gene) minimal promoter in conjunction with five IFN-stimulated response elements. The SEAP gene is driven by an IFN-β minimal promoter fused to five copies of the NF-kB response element. As a result, they allow the simultaneous study of the IFN regulatory factor (IRF)...
line modified for this purpose. This cell line is derived from HEK 293 (Human Embryonal Kidney) cells. It is stably transfected with the SEAP (secreted alkaline phosphatase) reporter gene under the transcriptional control of an NF-kB response element. The recombinant cell line is provided frozen...