Activation of interleukin-2 gene transcription via the T-cell surface molecule CD28 is mediated through an NF-B like response element. J. Biol. Chem. 266:14179-14182.Verweij, C. L., M. Geerts, and L. A. Aarden. 1991. Activation of interleukin-2 gene transcriptio...
Real-time monitoring of inflammation status in 3T3-L1 adipocytes possessing a secretory Gaussia luciferase gene under the control of nuclear factor-kappa B response element. Biochem Biophys Res Commun. 2012 Apr 13;420(3):623-7. doi: 10.1016/j.bbrc.2012.03.049. Epub 2012 Mar 17. ...
The SEAP gene is driven by an IFN-β minimal promoter fused to five copies of the NF-kB response element. As a result, they allow the simultaneous study of the IFN regulatory factor (IRF) and the NF-kB pathway by assessing the activity of Lucia luciferase and SEAP, respectively. Both ...
The TLR4 reporter cell line is a stably co-transfected cell line which expresses full-length human Toll-like receptor 4 (TLR4) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. The TLR4 reporter cell line has been validated...
driven by four copies of the NF-kB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-kB transcription factors bind to the DNA response elements, inducing transcription of the luciferase ...
NFkBResponsiveLuciferaseReporterHepG2StableCellLineisderivedfromhumanlivercancer,andstablyexpressfireflyluciferasereportergeneunderthecontroloftheNFkB responseelement. Thiscelllineisanidealcellularmodelformonitoringtheactivation ofNFkBReceptorSignalingPathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown....
(B) The sequence of the kB element in promoters, represented by blue and red boxes, within DNA may bind specific RHR family proteins. (C) The same kB element (red box) may bind more than one RHR dimer under different circumstances, leading to different transcriptional outcomes. (D) The ...
Description NF-κB TWO-Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor cell viability alongside NF-κB (nuclear factor Kappa B) activity. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element located upstream of the minimal...
The pGL4.32[luc2P/NF-κB-RE/Hygro] plasmid containing five copies of an NF-kB response element was purchased from Promega (Promega Corp., Madison, WI, USA). Luciferase activity was measured in samples containing equivalent amounts of protein using a luminometer and luciferase assay reagents. DP...