Real-time monitoring of inflammation status in 3T3-L1 adipocytes possessing a secretory Gaussia luciferase gene under the control of nuclear factor-kappa B response element. Biochem Biophys Res Commun. 2012 Apr 13;420(3):623-7. doi: 10.1016/j.bbrc.2012.03.049. Epub 2012 Mar 17. ...
NFkBResponsiveLuciferaseReporterMCF-7StableCellLineisderivedfromhumanbreastcancer,andstablyexpressfireflyluciferasereportergeneunderthecontroloftheNFkB responseelement. Thiscelllineisanidealcellularmodelformonitoringtheactivation ofNFkBReceptorSignalingPathwaytriggeredbystimulitreatment,enforcedgeneexpressionandgeneknockdown....
The TLR4 reporter cell line is a stably co-transfected cell line which expresses full-length human Toll-like receptor 4 (TLR4) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. The TLR4 reporter cell line has been validated...
driven by four copies of the NF-kB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-kB transcription factors bind to the DNA response elements, inducing transcription of the luciferase ...
Human Embryonic Kidney (HEK/293) cells were stably transfected with a vector carrying EGFP or d2EGFP under control of a synthetic promoter containing 4 copies of the NF-kB response element. Treatment with tumor necrosis factor 伪 (TNF-伪) gave rise to substantial EGFP/d2EGFP expression in up...
element located upstream of a minimal promoter. The NF-kB reporter is premixed with constitutively-expressing Renilla luciferase vector, whichserves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing ...
The pGL4.32[luc2P/NF-κB-RE/Hygro] plasmid containing five copies of an NF-kB response element was purchased from Promega (Promega Corp., Madison, WI, USA). Luciferase activity was measured in samples containing equivalent amounts of protein using a luminometer and luciferase assay reagents. DP...
(B) The sequence of the kB element in promoters, represented by blue and red boxes, within DNA may bind specific RHR family proteins. (C) The same kB element (red box) may bind more than one RHR dimer under different circumstances, leading to different transcriptional outcomes. (D) The ...
The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. Purchase of this ...