[3] Mircea Cretu Stancu, et al. Mapping and phasing of structural variation in patient genomes using nanopore sequencing. Nat Commun . 2017 Nov 6;8(1):1326. [4]Alison D. Tang, et al. Full-length transcript characterization of SF3B1mutation in...
文章名称:Full-length tranome analysis of asparagus roots reveals the molecular mechanism of salt tolerance induced by arbuscular mycorrhizal fungi 发表期刊:Environmental and Experimental Botany 发表时间:2021年1月 影响因子:4.027 研究内容:土壤盐分是最严重的非生物胁迫之一,限制了农业生产力和农作物在世界范围...
pychopper只有检测到双端的引物才能识别定义为全长完整(full length)的序列,所以在这之前不要对序列两端的引物(primers)进行裁剪去除,包括在用Dorado电信号转化碱基的时候。 通过-Q和-z可以实现chopper的功能,所以如果没有污染序列去除的话,可以跳过第二部直接使用pychopper。 -k 可以指定试剂盒和protocol。kit{PCS10...
This method can be applied in cell lines or tissue samples, enabling accurate detection of full-length circRNAs in the range of 100-3,000 bp. The entire protocol can be completed in 1 d, and can be scaled up for large-scale analysis using the nanopore barcoding kit and PromethION ...
Ultra-long sequencing Longest reads Best for: The most complete assemblies of large genomes Read N50s > 50kb, reads up to 4 Mbases Modified base information as standard RNA sequencing Full-length transcripts Best for: Single cell sequencing ...
1.Cuscó A, Catozzi C, Viñes J et al. Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and whole rrn operon 这篇文章采用了比较测16S和rrn序列(16S rRNA–ITS–23S rRNA; 4,500 bp),结果使用EPI2ME的话16S序列中只有68%的序列能够匹配到正确的分...
Unidirectional single-file transport of full-length proteins through a nanopore. Nat. Biotechnol. 41, 1130–1139 (2023). CAS PubMed PubMed Central Google Scholar Restrepo-Pérez, L. et al. Resolving chemical modifications to a single amino acid within a peptide using a biological nanopore. ...
The present study explored the association between gut-pharyngeal microbiome and TB stages in cynomolgus macaques using the full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. The total of 71 macaques was divided into TB () control, TB ( ) latent and TB ( ) active ...
We introduce a two-stage assembler to utilize the full length of nanopore reads. Our tool achieves superior performance in both error correction and de novo assembling nanopore reads. It requires only 8122 hours to assemble a 35X coverage human genome and achieves a 2.47-fold improvement in ...
2018. Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA. Proc Natl Acad Sci ... R Volden,T Palmer,A Byrne,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 0发表...