[3] Mircea Cretu Stancu, et al. Mapping and phasing of structural variation in patient genomes using nanopore sequencing. Nat Commun . 2017 Nov 6;8(1):1326. [4]Alison D. Tang, et al. Full-length transcript characterization of SF3B1mutation in...
文章名称:Full-length tranome analysis of asparagus roots reveals the molecular mechanism of salt tolerance induced by arbuscular mycorrhizal fungi 发表期刊:Environmental and Experimental Botany 发表时间:2021年1月 影响因子:4.027 研究内容:土壤盐分是最严重的非生物胁迫之一,限制了农业生产力和农作物在世界范围...
本研究表明,R10.4.1全长16S rRNA序列可用于环境微生物群的物种鉴定。 2023年10月6日,环境微生物领域业内权威期刊杂志Applied and Environmental Microbiology(简称AEM)在线发表了华中农业大学陈雯莉教授团队的研究成果,ONT 16S rRNA评测文章,文章题目为“The Newest Oxford Nanopore R10.4.1 Full-length 16S rRNA Seque...
植物全长RNA图谱揭示了Poly(A)长度的组织特异性和进化保守性 文章题目:An atlas of plant full-length RNA reveals tissue-specific and monocots-dicots conserved regulation of Poly(A) tail length 发表期刊:Nature Plants(IF=17.352) 发表时间:2022.08.18 研究内容 Poly(A)尾是真核生物mRNA的标志之一,在调节m...
These results provide a more complete data source of full-length transcriptome for the further study of gene expression and genetics in Muscovy duck. The hub genes and potential mechanisms that affect the ovulation of Muscovy duck have been screened out to provide a scientific basis for breeding ...
1.Cuscó A, Catozzi C, Viñes J et al. Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and whole rrn operon 这篇文章采用了比较测16S和rrn序列(16S rRNA–ITS–23S rRNA; 4,500 bp),结果使用EPI2ME的话16S序列中只有68%的序列能够匹配到正确的分...
pychopper只有检测到双端的引物才能识别定义为全长完整(full length)的序列,所以在这之前不要对序列两端的引物(primers)进行裁剪去除,包括在用Dorado电信号转化碱基的时候。 通过-Q和-z可以实现chopper的功能,所以如果没有污染序列去除的话,可以跳过第二部直接使用pychopper。
To enable a global comparison to the linear mRNA transcriptome, we performed ONT full-length poly(A) RNA sequencing on the same brain samples. This resulted in a total of 21.7 and 15.4 million reads for human and mouse brain-derived RNA samples, respectively. The FLAIR tool (Full-Length Alt...
pychopper只有检测到双端的引物才能识别定义为全长完整(full length)的序列,所以在这之前不要对序列两端的引物(primers)进行裁剪去除,包括在用Dorado电信号转化碱基的时候。 通过-Q和-z可以实现chopper的功能,所以如果没有污染序列去除的话,可以跳过第二部直接使用pychopper。
Nanopore sequencing is being applied in genome assembly, full-length transcript detection and base modification detection and in more specialized areas, such as rapid clinical diagnoses and outbreak surveillance. Many opportunities remain for improving data quality and analytical approaches through the ...