As the underlying mechanisms of DN development remains unclear, there is no ideal methods for the diagnosis and therapy. Objective: We aimed to explore the key genes and pathways that affect the rate progression of DN. Methods: Nanopore-based full-length transcriptome sequencing was performed with...
To enable a global comparison to the linear mRNA transcriptome, we performed ONT full-length poly(A) RNA sequencing on the same brain samples. This resulted in a total of 21.7 and 15.4 million reads for human and mouse brain-derived RNA samples, respectively. The FLAIR tool (Full-Length Alt...
et al. Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force. Nat Biotechnol. https://doi.org/10.1038/s41587-023-01954-x (2023). Zhang, Y. et al. Peptide sequencing based on host–guest interaction-assisted nanopore sensing. Nat. ...
Data for a 2D read of a full-length λ phage dsDNA from the MinION nanopore sequencer. a Steps in DNA translocation through the nanopore: (i) open channel; (ii) dsDNA with lead adaptor (blue), bound molecular motor (orange) and hairpin adaptor (red) is captured by the nanopore; capture...
Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans publication19 February 2024 Environmental DNA metabarcoding of freshwater fish in Malaysian tropical rivers using short-read nanopore sequencing as a potential biomonitoring too...
(Methods), and visually inspected them in IGV. These telomere-like repeat sequences could also be miscalled into repeat sequences of other repeat monomer length. For instance, we observed that the 6-mer (CCCTCA)nrepeats could get miscalled into the 5-mer (CCTCA)nrepeat sequence (...
Research releases are provided as technology demonstrators to provide early access to features or stimulate Community development of tools. Support for this software will be minimal and is only provided directly by the developers. Feature requests, improvements, and discussions are welcome and can be ...
Whether you are studying the human genome, investigating the microbiome, or exploring the diversity of life on earth, Oxford Nanopore library preparation solutions offer a flexible, cost-effective, and streamlined way to generate high-quality sequencing
tail length is stored in thept:itag of the output record. Reads for which the tail length could not be estimated will not have thept:itag. Custom primer sequences, estimation of interrupted tails, and plasmid support can be configured through the--poly-a-configoption. Seeherefor more ...
Ion transport and hydrodynamic flow through nanometer-sized channels (nanopores) have been increasingly studied owing to not only the fundamental interest in the abundance of novel phenomena that has been observed but also their promising application in innovative nanodevices, including next-generation sequ...