Thus, this new ONT method greatly improves base-calling accuracy, allowing for the de novo assembly of high-quality finished bacterial or plasmid genomes without the need for shortread sequencing. This saves both money and time and supports the application of ONT data in cr...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
文章主题:Transcriptome variation in human tissues revealed by long-read sequencing 发表杂志:Nature(IF=69.504) 发表时间:2022.08.03 研究内容 1.使用Nanopore全长转录组技术鉴定到人类70,000多个新转录本,并使用质谱验证蛋白表达 转录结构的调控产生了转录本的多样性,这在人类疾病中起着重要的作用。作者使用基于Nan...
Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for in...
Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformat
Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads. Here, we a
NanoPlot: Plotting tool for long read sequencing data and alignments. 对长度长测序和比对数据的统计报告进行可视化。 以上所有软件都存放在Nanopack的github里面,可以通过一行安装命令进行安装: #一键安装ONT系列质控软件$ pip install nanopack# NanoComp NanoFilt NanoLyse NanoPlot NanoStat ...
NanoPlot可以直接对guppy生成的sequencing_summary.txt的绘图。 Nanoplot --summary sequencing_summary.txt --loglength -o summary 选项参数: -t:线程数目 -o, --outdir:输出结果目录 -p, --prefix:输出结果前缀 --color:点的颜色 --N50 表示在序列读长的直方图中显示N50的标识 ...
Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for in...
全长转录组,又称为Isoform Sequencing(Iso-Seq),无需打断,基于三代测序平台直接获取转录本的5ˊ到3ˊ高质量全长序列,可准确识别可变剪接、基因融合、可选择性多聚腺苷酸化APA、等位基因特异性表达等转录本结构方面变异。基于Nanopore三代测序平台进行全长转录组测序,除了可准确鉴别上述转录本结构变异,还可实现转录本...