Whether you are studying the human genome, investigating the microbiome, or exploring the diversity of life on earth, Oxford Nanopore library preparation solutions offer a flexible, cost-effective, and streamlined way to generate high-quality sequencing
The use of a modified basecaller that corrects for ambiguous signal in homopolymeric stretches reduced the error rate for both library preparation methods to approximately 0.30%. Our study indicates that direct mtDNA sequencing from native DNA on the MinION device provides comparable results to those ...
Oxford Nanopore DNA sequencing Library preparation was carried out using the ligation sequencing kits (Oxford Nanopore Technologies) SQK-LSK109 and SQK-LSK112 for sequencing on R.9.4.1 and the R.10.4 flowcells, respectively. Anaerobic digester and Zymo R.9.4.1 datasets were generated on a MinION...
library preparation techniques (1D ligation and 1D rapid), sequencing speed (450 bases/s), and control software have increased throughput, so we hypothesized that whole-genome sequencing (WGS) of a human genome might be feasible using only a MinION nanopore sequencer17,18,19. ...
Library preparation and Oxford Nanopore sequencing The Oxford Nanopore Technology SQK-DCS108 kit was used for PCR-free direct cDNA sequencing. For each DCS108 library (CTR, UPF1, SMG6, SMG7, CTR, dKD), 500 ng of polyA+RNA was used as input, following the instructions of the ONT protocol...
Library for full-length transcripts sequencing cDNA sequencing Single-cell sequencing Base modifications preserved Direct RNAcDNA Direct RNA Sequencing Kit cDNA-PCR Sequencing Kit Preparation time 135 min ~225 mins + PCR Input recommendation 300 ng poly(A)+ RNA or 1 μg total RNA 10 ng poly(A)...
BaseClear offers a complete end-to-end nanopore sequencing workflow including sampling solutions, DNA extraction, library preparation to sequencing and analysis. In addition we provide consultancy and support for project design. Key applications include; Ultra-fast microbial genome sequencing: Within 5 bu...
Next Generation Sequencing Library Preparation Products 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 E7180S -20 NEBNext® FFPE DNA Repair Buffer E7181AVIAL -20 1 x 0.084 ml Not Applicable NEBNext® FFPE DNA Repair Mix E7182AVIAL -20 1 x 0.048 ml Not App...
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidd...
Furthermore, this study developed sophisticated library preparation and data analyses methods to enable high-accuracy analysis of amplicon libraries from complex microbial communities for sequencing on the nanopore platform. Besides, the best performing library preparation and data analyses methods were used...