Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for in...
Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformat
-i --input sequencing_summary.txt files produced by Guppy basecalles. Either a full path to a sequence_summary.txt file, or a full path to a directory containing one or more such files. In the latter case the directory is searched recursively. -s --output directory -p --processors Numb...
Individual writings include "NanoporeMethods for DNA Detection and Sequencing", "Engineering Gene Circuits: Foundations and Applications", "Nanotechnologies in Adult Stem Cell Research", and much more. Nanotechnology in Biology and Medicine Unrelated to the BioTrove collaboration, Applied Biosystems has in...
Our study indicates that direct mtDNA sequencing from native DNA on the MinION (TM) device provides comparable results to those obtained from common mtDNA sequencing methods and is a reliable alternative to approaches using PCR-enriched libraries.Zascavage, Roxanne R.Thorson, KelciePlanz, John V....
Nanopore sequencing is being applied in genome assembly, full-length transcript detection and base modification detection and in more specialized areas, such as rapid clinical diagnoses and outbreak surveillance. Many opportunities remain for improving data quality and analytical approaches through the ...
However, these methods lose information contained in biological RNA because the copied reads are often short and modifications are not retained. We address these limitations using a native poly(A) RNA sequencing strategy developed by Oxford Nanopore Technologies. Our study generated 9.9 million aligned...
The webinar did not cover RNA extraction methods. The Nanopore ARTIC workflow includes all steps from reverse transcription to the generation of sequencing reads and data analysis methods. Extraction and qPCR protocols will vary based on each i...
we here combined long-read Nanopore cDNA sequencing with short-read sequencing. We used the long reads to create a curated reference transcriptome, while the short reads were used to estimate the abundance of individual isoforms and identify those that respond to fluctuations in the levels of NMD...
lower costs and long reads of these methods. Nanopore applications, however, extend far beyond nucleic acid sequencing. In this Review, we present an overview of the broad applications of nanopores in molecular sensing and sequencing, chemical catalysis and biophysical characterization. We highlight th...