MinION is a nanopore sequencing platform, which provides long targeted DNA sequences. The long reads provide unambiguous phasing, but the initial high error profile prevented its use in high-impact applications such as Human Leukocyte Antigen (HLA) typing for HLA matching of donor and recipient in...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
Based on the innovative technology of Oxford Nanopore Technologies (ONT), BaseClear offers ultra fast and long-read sequencing services.
Nanopore sequencing Long-read Alternative splicing 1. Introduction CircRNAs are a group of RNAs defined by a covalent bond between the 5′ and 3′ end formed by a process known as back-splicing. The existence of these peculiar RNAs has been known since the seventies [1], [2] but it is ...
Ultra-deep, long-read nanopore sequencing of mock microbial community standards | Welcome to Oxford Nanopore technologies. Our goal is to enable the analysis of any living thing, by any person, in any environment
这推动了许多long-read sequencing策略的发展。两个最常用的商用技术就是: Pacific Biosciences’ Single Molecule Real-Time(SMRT)测序 平均读长~20kb HiFi读段>99.9%准确率 Oxford Nanopore Technologies’ nanopore测序 平均读长~100kb(ultral-long reads) R10.4 ~99%准确率 他们的原理都不相同,产生的数据的长度...
The long and the short of it: unlocking nanopore long-read RNA sequencing data with short-read tools 长和短:用短读工具解锁纳米孔长读RNA测序数据 View ORCID ProfileXueyiDong,View ORCID ProfileLuyiTian,View ORCID ProfileQuentinGouil,HasaruKariyawasam,View ORCID ProfileShianSu,View ORCID ProfileRicar...
Over the past decade, long-read, single-molecule DNA sequencing technologies have emerged as powerful players in genomics. With the ability to generate reads tens to thousands of kilobases in length with an accuracy approaching that of short-read sequencing technologies, these platforms have proven ...
SiLiCO: A Simulator of Long Read Sequencing in PacBio and Oxford Nanopore 硅:在PacBio和牛津纳米孔长读测序的模拟器 View ORCID ProfileEthan Alexander GarcíaBaker,SaraGoodwin,W. RichardMcCombie,View ORCID ProfileOliviaMendivil Ramos doi: https://doi.org/10.1101/076901 ...
Single-cell nanopore sequencing of full-length mRNAs transforms single-cell multi-omics studies. However, challenges include high sequencing errors and dependence on short-reads and/or barcode whitelists. To address these, we develop scNanoGPS to calculate same-cell genotypes (mutations) and phenotype...