结合加权基因共表达网络分析和转录本差异表达分析找到了参与初级生长、次级生长的关键转录因子。本项研究证明了Nanopore sequencing technology为调查种子植物器官发育提供了一种经济高效的方法。 原文链接:https://www.frontiersin.org/articles/10.3389/fgene.2021.615284/full 02 小RNA在种间异种嫁接植物芽到根单向移动 ...
Recently, a novel QitanTech nanopore long-read sequencing technology has emerged in China, but the potential application and performance were unexplored. Herein, we comprehensively evaluated the feasibility of the emerging sequencing technology in assembling complete genomes of MDR pathogens...
Nanopore测序是新一代基于纳米孔的单分子实时电信号测序技术,进行全长转录组测序。全长转录组,又称为Isoform Sequencing(Iso-Seq),无需打断,基于三代测序平台直接获取转录本的5ˊ到3ˊ高质量全长序列,可准确识别可变剪接、基因融合、可选择性多聚腺苷酸化APA、等位基因特异性表达等转录本结构方面变异。基于Nanopore三...
Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads. Here, we assess the performance of different methylation-callin...
话说前两天提到了Nature将长读长测序(long-read sequencing)确定为method of the year。 Pacbio欢欣鼓舞,Nanopore则保持了英伦绅士的矜持内涵。 今天,刚好ONT(Oxford Nanopore technology)发布了初步的2022年营收情况,那就谈谈他吧。 营收大增...
Based on the innovative technology of Oxford Nanopore Technologies (ONT), BaseClear offers ultra fast and long-read sequencing services.
Nanopore sequencing technology and tools for genome assembly: computational analysis of the current state, bottlenecks and future directions. Brief. Bioinform. 20, 1542–1559 (2019). Article PubMed Google Scholar Amarasinghe, S. L. et al. Opportunities and challenges in long-read sequencing data...
Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformat
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
F. Nanopore sequencing technology, bioinformatics and applications. Nat. Biotechnol. 39, 1348–1365 (2021). CAS PubMed PubMed Central Google Scholar Marx, V. Method of the year: long-read sequencing. Nat. Methods 20, 6–11 (2023). CAS PubMed Google Scholar Deamer, D., Akeson, M.,...