07 利用第三代纳米孔长读段测序技术构建和注释蜜蜂球囊菌的全长转录组 文章名称:Nanopore long-read tranome data of fungal pathogen of chalkbrood disease, Ascosphaera apis 预印:bioRxiv 研究内容:蜜蜂球囊菌(Ascosphaera apis,简称球囊菌)是一种专性侵染蜜蜂幼虫的真菌病原,导致的白垩病是严重影响养蜂生产的...
In the present work we illustrate the optimization of two sequencing protocols based on long-read Oxford Nanopore Technology to detect mtDNA structural alterations. This approach presents strong advantages in the analysis of mtDNA compared to both shortread NGS and traditional te...
下机数据共15.6G(Table 2),k-mer分析预估基因组~860 Mb,下机数据基本上是基因组18X测序深度。 Table 2 Nanopore测序 组装结果 研究者开发组装新工具TULIP(The Uncorrected Long-read Integration Process),在二代数据基础上组装得到基因组891.7 Mb,Contig N50为1.2M,相对已有短读长组装的基因组草图提升显著。©...
Nanopore long reads测序相对与传统二代测序short reads 的测序而言,对于可变剪切的分析具有巨大的优势。 简单来说该流程可以应用short reads RNA-seq对Nanopore long reads 的测序的结果进行校正,然后可以获得相对较为准确的各个isoform的表达信息,并可以对于该结果进行统计检验。 我使用的是SLURM 递交系统的HPC,我的的...
近日,由中南大学生殖与干细胞工程研究所林戈教授与北京希望组生物科技有限公司(以下简称“希望组”)梁帆带领的团队在BioRxiv在线发表一篇题为《Localization of balanced chromosome translocation breakpoints by long-read sequencing on the Oxford Nanopore platform》的研究成果。该研究利用Oxford Nanopore测序技术,从6个...
Nanopore测序原理：纳米孔测序，顾名思义，核心就是利用一个纳米孔，孔内共价结合有分子接头，将纳米孔...
Nanopore long-read sequencing is an emerging approach for studying genomes, including long repetitive elements like telomeres. Here, we report extensive basecalling induced errors at telomere repeats across nanopore datasets, sequencing platforms, basecallers, and basecalling models. We find that telomere...
Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include shor
Nanopore long-read sequencing technology offers promising alternatives to high-throughput short read sequencing, especially in the context of RNA-sequencing. However this technology is currently hindered by high error rates in the output data that affect analyses such as the identification of isoforms, ...
Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads. Here, we assess the performance of different methylation-callin...