Here, we use long-read nanopore sequencing of nicked circRNAs (circNick-LRS) and characterize a total of 18,266 and 39,623 circRNAs in human and mouse brain, respectively. We further develop an approach for tar
Circular reverse transcription and size selection achieves a 20-fold higher enrichment of circRNAs from total RNA compared to previous methods. We developed an algorithm, called circRNA identifier using long-read sequencing data (CIRI-long), to reconstruct the sequence of circRNAs. The workflow was ...
While different detection methods exist, the information of their full sequence remains more difficult to reveal, but is crucial to understand their function. Here, we describe a protocol to enrich for circRNAs that are subjected to long-read sequencing with the Oxford Nanopore long-read sequencing...
Full-length circRNA was sequenced in BNT162b2 mRNA vaccinated blood.Binding motifs of stress granule assemblies proteins enriched on sequenced circRNAs.SARS-CoV-2 related RNA binding proteins PABPC1, PUM1, YBX1.Long-read circRNA sequencing compared with short-read whole transcriptome sequencing....
The workflow was validated with simulated data and by comparison to Illumina sequencing as well as quantitative real-time RT鈥揚CR. We used CIRI-long to analyze adult mouse brain samples and systematically profile circRNAs, including mitochondria-derived and transcriptional read-through circRNAs. We ...
Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons.doi:10.1038/S41467-021-24975-ZKarim RahimiMorten T. VenDaniel M. DupontJrgen KjemsSpringer Science and Business Media LLC...
Circular RNAs (circRNAs) have important roles in regulating developmental processes and disease progression. As most circRNA sequences are highly similar to their cognate linear transcripts, the current short-read sequencing-based methods rely on the back-spliced junction signal for distinguishing circular...
The assembled full-length transcripts and their splicing patterns provide indispensable information to explore the biological function of circRNAs. The CIRI-long protocol combines circular reverse transcription and long-read nanopore sequencing to capture full-length circular RNA isoforms, allowing their ...
Third-generation (long-read) sequencingColorectal cancerGene expression regulationCircular RNAs (circRNAs) constitute an RNA type formed by back-splicing. BCL2-like 12 ( BCL2L12 ) is an apoptosis-related gene comprising 7 exons. In this study, we used targeted nanopore sequencing to identify ...