Leonardi, T., Leger, A. (2021). Nanopore RNA Sequencing Analysis. In: Picardi, E. (eds) RNA Bioinformatics. Methods in Molecular Biology, vol 2284. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1307-8_31 Download citation...
21号,BASE团队在Nature Communications发表文章:mRNA vaccine quality analysis using RNA sequencing,他们开发了一种采用长读长纳米孔测序分析 mRNA 疫苗关键质量属性的方法,称为 VAX-seq,对 mRNA 序列,长度,完整度,纯度,修饰核苷展开精确定量分析。相比现有昂贵耗时的分析方法(HPLC、毛细管电泳、凝胶电泳等),VAX-seq ...
This chapter describes MasterOfPores v.2 (MoP2), an open-source suite of pipelines for processing and analyzing direct RNA Oxford Nanopore sequencing data. The MoP2 relies on the Nextflow DSL2 framework and Linux containers, thus enabling reproducible data analysis in transcriptomic and ...
21号,BASE团队在Nature Communications发表文章:mRNA vaccine quality analysis using RNA sequencing,他们开发了一种采用长读长纳米孔测序分析 mRNA 疫苗关键质量属性的方法,称为 VAX-seq,对 mRNA 序列,长度,完整度,纯度,修饰核苷展开精确定量分析。相比现有昂贵耗时的分析方法(HPLC、毛细管电泳、凝胶电泳等),VAX-seq序...
Nanopore native RNA sequencing of a human poly(A) transcriptome FLAIR原始文章 FLAIR分析流程 FLAIR分析流程示意图 Nanopore long reads测序相对与传统二代测序short reads 的测序而言,对于可变剪切的分析具有巨大的优势。 简单来说该流程可以应用short reads RNA-seq对Nanopore long reads 的测序的结果进行校正,然后...
这里我们的示例是来自毛叶杨的纳米孔测序数据[2],是对RNA进行的测序工作。数据集位置SRR8491764(GridION sequencer),然后找到其SRP182578,对其原始文件进行下载 如何从NCBI下载原始数据文件,参考:生物信息学入门——NCBI SRA入门原始下机数据对做甲基化的可能更有帮助一些,对SV等工作可能只需要下载fastq格式的序列文件即...
文章名称:Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome 发表期刊:Plant Method 发表时间:2020年4月 影响因子:3.610 研究内容:在许多不同的研究领域中,使用Pacifc Biosciences(PacBio)和牛津纳米孔技术(ONT)进行第三代测序的研究数量正在迅速增加。
文章名称:Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis tranome 发表期刊:Plant Method 发表时间:2020年4月 影响因子:3.610 研究内容:在许多不同的研究领域中,使用Pacifc Biosciences(PacBio)和牛津纳米孔技术(ONT)进行第三代测序的研究数量正在迅速增加。与...
Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been difficult in part because a simple method to simultaneously quantify their abundance and chemical modifications is lacking. Here we introduce Nano-tRNAseq, a nanopor
The advent of portable nanopore sequencing devices has enabled DNA and RNA sequencing to be performed in the field or the clinic. However, advances in in situ genomics require parallel development of portable, offline solutions for the computational anal