We address these limitations using a native poly(A) RNA sequencing strategy developed by Oxford Nanopore Technologies. Our study generated 9.9 million aligned sequence reads for the human cell line GM12878, using thirty MinION flow cells at six institutions. These native RNA reads had a median ...
We address these limitations using a native poly(A) RNA sequencing strategy developed by Oxford Nanopore Technologies. Our study generated 9.9 million aligned sequence reads for the human cell line GM12878, using thirty MinION flow cells at six institutions. These native RNA reads had a median ...
Nanopore native RNA sequencing of a human poly(A) transcriptome FLAIR原始文章 FLAIR分析流程 FLAIR分析流程示意图 Nanopore long reads测序相对与传统二代测序short reads 的测序而言,对于可变剪切的分析具有巨大的优势。 简单来说该流程可以应用short reads RNA-seq对Nanopore long reads 的测序的结果进行校正,然后...
Nanopore native RNA sequencing of a human poly(A) transcriptome. Nature Methods doi: doi:10.1038/s41592-019-0617-2 rel2 [已修复] [2020年12月更新] 基序识别(Guppy 4.2.2) 完整的原生 RNA 数据集(30 次运行)、完整的 cDNA 数据集(12 次运行)和 IVT RNA 数据集。使用 Guppy 4.2.2 翻转(hac)...
[1] Garalde D R, Snell E A, Jachimowicz D, et al. Highly parallel direct RNA sequencing on an array of nanopores. Nature Methods,2018. [2] Workman, R.E. et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. bioRxiv (2018)....
Nanopore native RNA sequencing of a human poly(A) transcriptome Nat. Methods, 16 (2019), pp. 1297-1305, 10.1038/s41592-019-0617-2 View in ScopusGoogle Scholar 18 L.P. Vu, B.F. Pickering, Y. Cheng, S. Zaccara, D. Nguyen, G. Minuesa, T. Chou, A. Chow, Y. Saletore, M. Ma...
Oxford Nanopore sequencing Legacy short-read sequencing Streamlined, automatable workflows Laborious workflows Prepare samplesin as little as 10 minutes, including multiplexing Use end-to-endwhole-genome,metagenomic,targeted(including 16S barcoding),direct RNA and cDNAsequencing workflows ...
Workman RE, Tang AD, Tang PS, Jain M, Tyson JR, Razaghi R, et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. Nat Methods. 2019;16(12):1297–305.. CASPubMedPubMed CentralGoogle Scholar Colombo M, Karousis ED, Bourquin J, Bruggmann R, Mühlemann O. Transcriptom...
Application of host-depleted nanopore metagenomic sequencing in the clinical detection of pathogens in pigs and cats publication2 January 2024 Air monitoring by nanopore sequencing publication22 December 2023 Related areas Nanopore sequencing accuracyData analysis resourcesHuman genomicsPopulation genomicsGenome ...
E. et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. Nat. Methods 16, 1297–1305 (2019). CAS PubMed PubMed Central Google Scholar Abu-Shumays, R. L. et al. Direct nanopore sequencing of individual full length tRNA strands. ACS Nano 15, 16642–16653 (2021). ...