We address these limitations using a native poly(A) RNA sequencing strategy developed by Oxford Nanopore Technologies. Our study generated 9.9 million aligned sequence reads for the human cell line GM12878, using thirty MinION flow cells at six institutions. These native RNA reads had a median ...
Nanopore native RNA sequencing of a human poly(A) transcriptome FLAIR原始文章 FLAIR分析流程 FLAIR分析流程示意图 Nanopore long reads测序相对与传统二代测序short reads 的测序而言,对于可变剪切的分析具有巨大的优势。 简单来说该流程可以应用short reads RNA-seq对Nanopore long reads 的测序的结果进行校正,然后...
Nanopore native RNA sequencing of a human poly(A) transcriptome. Nature Methods doi: doi:10.1038/s41592-019-0617-2 rel2 [已修复] [2020年12月更新] 基序识别(Guppy 4.2.2) 完整的原生 RNA 数据集(30 次运行)、完整的 cDNA 数据集(12 次运行)和 IVT RNA 数据集。使用 Guppy 4.2.2 翻转(hac)...
G. d., Jones, K., Snutch, T. P., Loman, N. J., Paten, B., Loose, M. W., Simpson, J. T., Olsen, H. E., Brooks, A. N., Akeson, M., and Timp, W. (2018). Nanopore native RNA sequencing of a human poly(A) transcriptome. bioRxiv, page 459529....
E. et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. Nat. Methods 16, 1297–1305 (2019). CAS PubMed PubMed Central Google Scholar Abu-Shumays, R. L. et al. Direct nanopore sequencing of individual full length tRNA strands. ACS Nano 15, 16642–16653 (2021). ...
E. et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. Nat. Methods 16, 1297–1305 (2019). Article CAS PubMed PubMed Central Google Scholar Soneson, C. et al. A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes. ...
[1] Garalde D R, Snell E A, Jachimowicz D, et al. Highly parallel direct RNA sequencing on an array of nanopores. Nature Methods,2018. [2] Workman, R.E. et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. bioRxiv (2018)....
Fully characterise human genomic, epigenomic, and transcriptomic variation with real-time nanopore sequencing. Resolve complex regions, uncover previously hidden variation, and identify base modifications as standard with any-length reads. Generate highl
E. et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. Nat. Methods 16, 1297–1305 (2019). Article CAS PubMed PubMed Central Google Scholar Soneson, C. et al. A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes. ...
mutant named R9.4), library preparation techniques (1D ligation and 1D rapid), sequencing speed (450 bases/s), and control software have increased throughput, so we hypothesized that whole-genome sequencing (WGS) of a human genome might be feasible using only a MinION nanopore sequencer17,18,...