近年来新兴的纳米孔测序技术能够对长链RNA直接测序（nanopore direct RNA-sequencing），无需 PCR 扩增，可以同时在核苷酸序列中识别碱基修饰【3,4】。2021年7月19日，来自于新加坡A*STAR基因研究所的Jonathan Göke 研究组在Nature Biotechnology杂志上发表了题为Identification of differential RNA modifications from ...
while Nanopore direct RNA sequencing has the ability to detect such remote interactions. References:Workman R E, Tang A D, Tang P S, et al. Nanopore native RNA sequencing of a human poly (A) transcriptome[J]
Quantitative profiling of N 6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing[J]. Genome biology, 2021, 22: 1-17. ^Kovaka S, Hook P W, Jenike K M, et al. Uncalled4 improves nanopore DNA and RNA modification...
近年来新兴的纳米孔测序技术能够对长链RNA直接测序(nanopore direct RNA-sequencing),无需 PCR 扩增,可以同时在核苷酸序列中识别碱基修饰【3,4】。 2021年7月19日,来自于新加坡A*STAR基因研究所的Jonathan Göke研究组在Nature Biotechnology...
21号,BASE团队在Nature Communications发表文章:mRNA vaccine quality analysis using RNA sequencing,他们开发了一种采用长读长纳米孔测序分析 mRNA 疫苗关键质量属性的方法,称为 VAX-seq,对 mRNA 序列,长度,完整度,纯度,修饰核苷展开精确定量分析。相比现有昂贵耗时的分析方法(HPLC、毛细管电泳、凝胶电泳等),VAX-seq ...
21号,BASE团队在Nature Communications发表文章:mRNA vaccine quality analysis using RNA sequencing,他们开发了一种采用长读长纳米孔测序分析 mRNA 疫苗关键质量属性的方法,称为 VAX-seq,对 mRNA 序列,长度,完整度,纯度,修饰核苷展开精确定量分析。相比现有昂贵耗时的分析方法(HPLC、毛细管电泳、凝胶电泳等),VAX-seq序...
However, the dependence on cDNA in most RNA sequencing technologies restricts their ability to detect RNA base modifications. To address this limitation, the latest Oxford Nanopore Direct RNA Sequencing (ONT DRS) technology was employed to investigate the transcriptome of maize seedling roots under ...
would like to remove pcr bias are exploring attributes of native rna such as modified bases please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd party materials" tab for more detail. the direct rna sequencing kit features: feature property ...
2021年7月19日,来自于新加坡A*STAR基因研究所的Jonathan Göke研究组在Nature Biotechnology杂志上发表了题为Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore的论文,开发了一种基于nanopore direct RNA-seq的RNA修饰差异化分析计算方法xPore。xPore可以实现单碱基水平(...
以上数据表明,纳米孔RNA直接测序鉴定m6A修饰的效果优异。原文:DANIEL A. LORENZ, et al. Direct RNA sequencing enablesm6A detection in endogenous transcript isoforms at base-specific resolution. RNA(2020) 26:19–28. 广州表观生物科技有限公司 扫码关注 快捷导航 科学顾问 公司介绍 联系我们 联系方式: 020-...